ES-E14TG2a

08021401, mouse embryo (blastocyst). Monolayer of spheroidal cells on feeder layer of PMEFs or STOs

• Sinónimos: E14TG2a Cell Line
• Código UNSPSC: 41106514
• origen biológico: mouse embryo (blastocyst)
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
• productos: Not specified
• receptores: Not specified

Propiedades

• Nombre del producto: ES-E14TG2a, 08021401, from mouse embryo(blastocyst)
• origen biológico: mouse embryo (blastocyst)
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Adherent monolayer of spheroidal cells on feeder layer of mouse primary embryonic fibroblasts or STO cells. The cells spontaneously differentiate and form embryonal structures in the absence of a feeder layer.
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice

Descripción

Origen línea celular

Mouse (strain 129/Ola) embryonic stem cell

Descripción línea celular

Pluripotent mouse embryonic stem cell line. ES-E14TG2a cells are deficient in in HPRT and are resistant to 0.06mM 6-thioguanine.

Perfil del ADN

Not specified

Medio de cultivo

DMEM + 2 mM Glutamine + 1% NEAA + 0.1 mM 2-Mercaptoethanol + 1000 U/ml mouse inhibitory factor (LIF) + 1.5% sodium bicarbonate + 10% Fetal Bovine Serum. Renewal of medium every 2-3 days is required.

Rutina de subcultivo

Not specified

Otras notas

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