SNL 76/7 Cell Line from mouse

7032801, mouse esophagus, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• Nombre del producto: SNL 76/7 Cell Line from mouse, 07032801
• origen biológico: mouse esophagus
• descripción: Mouse SIM strain embryonic fibroblast
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Mouse SIM strain embryonic fibroblast

Descripción línea celular

SNL 76/7 clonally-derived from a STO cell line that expresses both G418 resistance and leukaemic inhibitory factor (LIF). The STO cell line was transfected with a G418-resistance cassette, RV4.0 (Thomas and Capecchi 1987), and a LIF expression construct (Williams et al., 1988). This cell line can be used as a feeder layer to support the growth of mouse embryonic stem cells. Inclusion of G418 in the media is not necessary for normal cell growth.

Medio de cultivo

Knockout D-MEM (Invitrogen) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Split confluent cultures 1:5-1:15 i.e. seeding at 2-4x10,000 cells/cm² using trypsin/EDTA;5% CO₂; 37°C.

Otras notas

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