VP303

5092806, human breast, Epithelial

• Código UNSPSC: 41106514

Propiedades

• product name: VP303, 05092806
• origen biológico: human breast
• modo de crecimiento: Adherent
• cariotipo: Mean no. 64, modal no. 67, range 57-72
• morfología: Epithelial
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• enfermedades relevantes: cancer
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Human Breast Cancer

Descripción línea celular

Derived from a breast cancer biopsy removed from a 69 year-old female. The histology of the breast cancer biopsy was defined as Ductal Grade II, 90% of tumour cells stained positive for oestrogen receptors and cells were negative for progesterone receptor staining. The VP303 cell line derived from the tumour did not stain positive for oestrogen receptors. The individual was recorded to be alive 5 years after surgery performed to remove the tumour. This cell line grows slowly and is relatively difficult to culture; please read the sub-culture routine information closely.

Medio de cultivo

MEBM (Clonetics, CC-3151) + MEGM SingleQuots (Clonetics, CC-4136) + 2mM Glutamine + 2% Foetal Bovine Serum (FBS). Fresh EGF (final concentration of 10ng/ml) should be added to the medium at each subculture.

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 2-5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. The VP series of breast cancer cell lines are very slow growing and relatively difficult to grow. Fresh EGF (final concentration 10ng/ml) s

Otras notas

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