Sf9 TitreHigh AC free

5011001, insect ovary, Spherical

• Código UNSPSC: 41106514

Propiedades

• product name: Sf9 TitreHigh AC free, 05011001
• origen biológico: insect ovary
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Spherical
• productos: Not specified
• receptores: Not specified
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Spodoptera frugiperda pupal ovarian tissue, serum-free

Descripción línea celular

This cell line is a derivative of the Sf9 cell line which has been adapted to TiterHigh Sf Insect Medium (Sigma I5408). TiterHigh Sf Insect Medium is an animal component-free, protein-free formulation designed specifically for the Sf9 (Spodoptera frugiperda) insect cell line. The cell line Sf9 is derived from pupal ovarian tissue of spodoptera frugiperda. The Sf9 cell line is highly susceptible to Baculovirus infection and is used in the production of protein products genetically manipulated into Baculovirus vector systems.

Aplicación

Virus studies: Baculovirus

Medio de cultivo

TiterHigh^™ Sf Insect Medium (Sigma I5408) no supplements.

Rutina de subcultivo

Maintain cultures between 3x100,000 to 1x1,000,000 cells/ml; 27°C. Gently resuspend cells in old culture medium by dislodging the cells by pipetting, or gentle agitation, and resuspend in fresh medium. Cells should stick to the culture flask. Freeze in 7.

Otras notas

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Información legal

TiterHigh is a trademark of Sigma-Aldrich Co. LLC