IIC9 (CH0)

30814, hamster embryo, Fibroblast

• Código UNSPSC: 41106514

Propiedades

• product name: IIC9 (CH0), 00030814
• origen biológico: hamster embryo
• modo de crecimiento: Adherent
• cariotipo: Not specified
• morfología: Fibroblast
• productos: Not specified
• receptores: PAR-1 (protease-activated receptor 1, thrombin receptor), EGF, PDGF
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Origen línea celular

Chinese hamster embryo fibroblast

Descripción línea celular

The Chinese hamster embryo fibroblast (CHEF) line is a nontumorigenic and stable diploid cell line, with a high serum requirement for growth. It is isolated from embryo preparation and is a spontaneous tumorigenic anchorage-independent line.

Aplicación

IIC9 (CH0) is suitable for selective growth of some rodent epithelial cells in a medium containing citrulline.

Medio de cultivo

DMEM:F12 (1:1) (D8062) + 5% FBS / FCS (F2442) + 2mM L-Glutamine (G7513).

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:10 ie. seeding at 2-4 x10,000 cells/cm² using 0.25% trypsin /EDTA; 37°C; 5% CO₂.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.