C9743
Anti-CNPase antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Sinónimos:
Anti-2′,3′-Cyclic nucleotide 3′-phosphodiesterase, Anti-CNP1
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About This Item
conjugado:
unconjugated
application:
FACS
IHC
WB
IHC
WB
clon:
polyclonal
reactividad de especies:
human, mouse, rat
citations:
10
técnicas:
flow cytometry: 1:200-1:500
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000
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origen biológico
rabbit
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
Formulario
buffered aqueous solution
mol peso
antigen 46-48 kDa
reactividad de especies
human, mouse, rat
concentración
~1 mg/mL
técnicas
flow cytometry: 1:200-1:500
immunohistochemistry: 1:100-1:200
western blot: 1:500-1:1,000
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CNP(1267)
mouse ... Cnp(12799)
rat ... Cnp(25275)
Categorías relacionadas
Inmunógeno
synthetic peptide corresponding to residues 122-136 of human CNPase. The sequence is 93% identical in mouse and rat.
Aplicación
Anti-CNPase antibody produced in rabbit is suitable for flow cytometry at a working dilution of 1:200-1:500, immunohistochemistry at 1:100-1:200 and western blotting at 1:500-1:1000. It was used at a working dilution of 1:1000 as a primary antibody in western blotting of cerebrospinal fluid samples in a study.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western Blotting (1 paper)
Acciones bioquímicas o fisiológicas
CNPase (2′,3′-cyclic nucleotide 3′-phosphodiesterase) is associated with brain tubulin and acts as a microtubule-associated protein in promoting microtubule assembly and distribution. It is expressed primarily in committed oligodendroglial progenitors and terminally-differentiated oligodendrocytes and may be used as a marker of cell differentiation in the CNS.
Forma física
solution in phosphate buffered saline, containing 0.02% sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Producto relacionado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
T Ishii et al.
Neuroscience letters, 268(3), 131-134 (1999-07-16)
We have previously reported that human amniotic epithelial (HAE) cells expressed neuronal and glial cell markers using immunostaining and western blotting. To study the expression system of these cell markers in HAE cells, we investigated the expression of mRNA for
Irum Naureen et al.
Fluids and barriers of the CNS, 10(1), 34-34 (2013-12-20)
In hydrocephalus an imbalance between production and absorption of cerebrospinal fluid (CSF) results in fluid accumulation, compression and stretching of the brain parenchyma. In addition, changes in CSF composition have a profound influence on the development and function of the
2',3'-cyclic nucleotide-3'-phosphohydrolase and signal transduction in central nervous system myelin.
R J Thompson
Biochemical Society transactions, 20(3), 621-626 (1992-08-01)
A J Douglas et al.
Annals of human genetics, 56(Pt 3), 243-254 (1992-07-01)
Human brain cDNA clones for the myelin associated enzyme 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase) have been isolated and sequenced. The only 5' untranslated region (UTR) sequence found was that of a human CNPII mRNA, with no direct evidence for a CNPI
S M Staugaitis et al.
Journal of neuroscience research, 25(4), 556-560 (1990-04-01)
The 46 kD isoform of the 2'3'-cyclic nucleotide 3'-phosphodiesterase (CNPI) was expressed in HeLa cells by transfection of its cDNA clone. The distribution of this polypeptide as mapped by indirect immunofluorescence and conventional epifluorescence microscopy appeared diffuse and generally uniform
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