C9358
Anti-Chk1 antibody, Mouse monoclonal
~2 mg/mL, clone DCS-310, purified from hybridoma cell culture
Sinónimos:
Anti-CHK1
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About This Item
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified from hybridoma cell culture
tipo de anticuerpo
primary antibodies
clon
DCS-310, monoclonal
formulario
buffered aqueous solution
mol peso
antigen 54 kDa
reactividad de especies
human
concentración
~2 mg/mL
técnicas
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells
isotipo
IgG2b
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CHEK1(1111)
Descripción general
Checkpoint kinase 1 (Chk1) protein is a serine/threonine-protein kinase with a molar mass of 54 kDa. Monoclonal Anti-Chk1 (mouse IgG2b isotype) is derived from the DCS-310 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with a recombinant human Chk1.
Inmunógeno
recombinant human Chk1.
Aplicación
Monoclonal Anti-Chk1 antibody produced in mouse has been used in:
- immunoprecipitation
- immunohistochemistry
- immunoblotting
Monoclonal Anti-Chk1 antibody produced in mouse is suitable for immunohistochemistry (formalin-fixed, paraffin-embedded sections), immunoprecipitation, microarray and western blot at a working concentration of 1-2 μg/mL using whole extract of cultured 293T (human embryonal kidney) cells. It was used for immunohistochemistry in a study to investigate the specific role of Chk1 phosphorylations in cell survival and checkpoint activation. It was used for immunohistochemistry at a working dilution of 1:4000 in a study to explore Chk1 knockdown as a novel therapeutic approach to arrest cell-cycle progression in MM cells, thus increasing the rate of cell death. It was used for the immunoprecipitation and detection of total Chk1 by western blot to study a possible regulatory relationship between Chk1 and CK1, a member of the casein kinase 1 family.
Acciones bioquímicas o fisiológicas
Checkpoint kinase 1 (Chk1) protein acts as a cell cycle checkpoint regulator.
The protein propagates signals from damaged or unreplicated DNA. It is expressed and active only in S-G2 phases of the cell cycle. In response to DNA damage, it phosphorylates CDC25C at Ser 216, thus preventing activation of the Cdc2-cyclin B complex and mitotic entry, blocking cell cycle progression. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Chk1 can phosphorylate Wee1, a negative regulator of G2 to M transition.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
C Lukas et al.
Cancer research, 61(13), 4990-4993 (2001-06-30)
The Chk2 kinase is a tumor suppressor and key transducer of DNA-damage checkpoints. We show that the human Chk2 protein is relatively stable, nuclear, and responding to gamma-radiation throughout the cell cycle. Contrary to the retinoblastoma protein-regulated, labile Chk1 kinase
Identification of potential therapeutic targets in malignant mesothelioma using cell-cycle gene expression analysis
Romagnoli S, et al.
The American Journal of Pathology, 174(3), 762-770 (2009)
Solange Romagnoli et al.
The American journal of pathology, 174(3), 762-770 (2009-02-17)
Cell-cycle defects are responsible for cancer onset and growth. We studied the expression profile of 60 genes involved in cell cycle in a series of malignant mesotheliomas (MMs), normal pleural tissues, and MM cell cultures using a quantitative polymerase chain
Specific role of Chk1 phosphorylations in cell survival and checkpoint activation
Niida H, et al.
Molecular and Cellular Biology, 27(7), 2572-2581 (2007)
Joachim Bischof et al.
PloS one, 8(7), e68803-e68803 (2013-07-19)
CK1δ, a member of the casein kinase 1 family, is involved in the regulation of various cellular processes and has been associated with the pathophysiology of neurodegenerative diseases and cancer. Therefore recently, interest in generating highly specific inhibitors for personalized
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