C9301

Chicken Collagen Type II

from chicken sternal cartilage, powder, suitable for cell culture

• Número de CAS: 9007-34-5
• Número CE: 232-697-4
• Número MDL: MFCD00130825
• Código UNSPSC: 12352202
• NACRES: NA.75

Propiedades

• product name: Collagen from chicken sternal cartilage, Type II (Miller), powder, BioReagent, suitable for cell culture
• origen biológico: chicken (Sternal cartilage)
• Nivel de calidad: 300
• tpo: Type II (Miller)
• Línea del producto: BioReagent
• formulario: powder
• envase: glass bottle of 100 mg; poly bottle of 25 mg; glass bottle of 5 mg
• concentración: 60-80% (biuret)
• técnicas: cell culture | mammalian: suitable
• cobertura de la superficie: 6‑10 μg/cm²
• solubilidad: acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
• Nº de acceso UniProt: P02460
• Especificidad de unión: Peptide Source: Fibrinogen
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: chicken ... COL2A1(395069)

Descripción

Descripción general

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Aplicación

Collagen from chicken sternal cartilage has been used:

• in enzyme–linked immunosorbent assay (ELISA).
• chondrocyte-mediated tissue production in vitro.
• induction, treatment, and assessment of collagenα induced arthritis (CIA).
• cell proliferation assay.
• as a coating for cell culture surfaces.

Acciones bioquímicas o fisiológicas

Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

Características y beneficios

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.

Otras notas

Major component of articular cartilage.

Nota de preparación

Prepared by a modification of the method of Trentham, D.E., et al.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)