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Merck

C9205

Sigma-Aldrich

Anti-proteína gliofibrilar ácida (GFAP) monoclonal antibody produced in mouse

clone G-A-5, purified from hybridoma cell culture

Sinónimos:

Anti-GFAP

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

Nivel de calidad

conjugado

CY3 conjugate

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

G-A-5, monoclonal

formulario

buffered aqueous solution

reactividad de especies

human, pig, rat

técnicas

direct immunofluorescence: 1:400 using alcohol-fixed, paraffin-embedded rat brain sections

isotipo

IgG1

Nº de acceso UniProt

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... GFAP(2670)
rat ... Gfap(24387)

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Descripción general

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 is a purified mouse monoclonal antibody conjugated to Cy3 reactive dye. It is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. GFAP, in the central nervous system, is found in developing, normal, reactive and neoplastic astrocytes and ependymal cells. It is expressed in developing oligodendrocytes in the peripheral nervous system. GFAP is found in some Schwann cells, enteric glial cells, Müller glia of the retina, and the satellite cells of human sensory ganglia. It has been demonstrated in some non-glial cells as well.

Especificidad

El anticuerpo reacciona específicamente con la GFAP en análisis de inmunotransferencia y marca los astrocitos, las células de la glía de Bergmann y los condrocitos del cartílago elástico en la tinción inmunohistoquímica. El anticuerpo reacciona con el antígeno glial específico en cortes de tejido congelados o fijados en alcohol.
The antibody reacts specifically with GFAP in immunoblotting assays and labels astrocytes, and chondrocytes of elastic cartilage in immunohistochemical staining. The antibody reacts with glial specific antigen in frozen or alcohol-fixed tissue sections.

Inmunógeno

GFAP de médula espinal de cerdo
GFAP from pig spinal cord

Aplicación

Monoclonal Anti-Glial Fibrillary Acidic Protein (GFAP) (mouse IgG1 isotype) conjugated to Cy3 has been used in
  • immunohistochemistry
  • immunoblotting
  • immunolabelling,
  • immunocytochemistry
  • immunofluorescence

Acciones bioquímicas o fisiológicas

Glial Fibrillary Acidic Protein (GFAP) is expressed in several glial-related intracranial neoplasms such as astrocytomas and to a variable extent in ependymomas, medulloblastomas, choroid plexus papillomas, gangliogliomas, mixed gliomas, hemangioblastomas, pineocytomas, pineoblastomas, pituitary adenomas, retinoblastomas, oligodendrogliomas and in a small minority of peripheral nerve sheath tumors (schwannomas and neurofibromas). It may also be found in pleomorphic adenomas and ovarian and testicular teratomas.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% BSA and 15 mM sodium azide

Información legal

Cy is distributed under license from Amersham Biosciences Limited.
Cy3 is a trademark of Cytiva

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Producto relacionado

anticuerpo

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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D B Pettigrew et al.
BMC neuroscience, 2, 9-9 (2001-06-12)
Brain and spinal cord white matter can support extensive axonal growth. This growth is generally constrained to an orientation that is parallel to the longitudinal axis of the fiber tract. This constraint is presumably due to permissive and non-permissive substrates
Sequel of spontaneous seizures after kainic acid-induced status epilepticus and associated neuropathological changes in the subiculum and entorhinal cortex
Drexel M, et al.
Neuropharmacology, 63(5), 806-817 (2012)
Aixa F Rivera-Pagán et al.
PloS one, 10(4), e0125195-e0125195 (2015-04-18)
Excitotoxicity due to glutamate receptor over-activation is one of the key mediators of neuronal death after an ischemic insult. Therefore, a major function of astrocytes is to maintain low extracellular levels of glutamate. The ability of astrocytic glutamate transporters to
Fibroblasts can express glial fibrillary acidic protein (GFAP) in vivo
Hainfellner JA, et al.
Journal of Neuropathology and Experimental Neurology, 60(5), 449-461 (2001)
Expression of vimentin and glial fibrillary acidic protein in central nervous system development of rats
Luo H, et al.
Asian Pacific Journal of Tropical Medicine, 10(12), 1185-1189 (2017)

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