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Merck

C7988

Sigma-Aldrich

Anti-Cre antibody, Mouse monoclonal

clone 7-23, purified from hybridoma cell culture

Sinónimos:

Mouse Anti-Recombinase cre

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.56

origen biológico

mouse

Nivel de calidad

conjugado

unconjugated

forma del anticuerpo

purified from hybridoma cell culture

tipo de anticuerpo

primary antibodies

clon

7-23, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~38 kDa

concentración

~2 mg/mL

técnicas

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
western blot: 0.5-1 μg/mL using recombinant Cre recombinase

isotipo

IgG1

Condiciones de envío

wet ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Descripción general

Monoclonal Anti-Cre (mouse IgG1 isotype) is derived from the 7-23 hybridoma produced by the fusion of mouse myeloma cells (X63-Ag8.653) and splenocytes from BALB/c mice immunized with Cre protein. Cre recombinase is a member of the integrase family of proteins that are site-specific recombinases.The protein is expressed in P1 bacteriophage.

Aplicación

Anti-Cre antibody ,Mouse monoclonal has been used in:
  • enzyme linked immunosorbent assay (ELISA)
  • immunoblotting
  • immunoprecipitation
  • immunohistochemistry
  • immunocytochemistry
  • flow cytometry
  • immunostaining

Acciones bioquímicas o fisiológicas

Cre recombinase enables DNA recombination between two recognition sites called loxP. Each one of these sites (34 bp long) contain two 13 bp palindromic flanking sequences and in between them is a core spacer sequence 8 bp long. The recombination event takes place within the spacer area of the two loxP sites. Cre/loxP system manipulates genes in mammalian systems by making deletions, gene replacements, insertions (knockin), conditional gene targeting (knockout), and point mutations. Monoclonal antibodies to the Cre protein are an important tool for the identification of Cre recombinase in different mammalian systems.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Almacenamiento y estabilidad

For continuous use, store at 2-8 °C for up to one month. For extended storage, the solution should be frozen in working aliquots. Repeated freezing and thawing is not recommended. Storage in "frost-free" freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

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Directional resolution of synthetic holliday structures by the Cre recombinase
Lee L and Sadowski PD
Test, 276(33), 31092-31098 (2001)
Juan D Rodriguez et al.
Genetics, 207(1), 129-138 (2017-07-12)
Transvection is broadly defined as the ability of one locus to affect its homologous locus
Katsuhisa Matsuura et al.
The Journal of cell biology, 167(2), 351-363 (2004-10-20)
The concept of the plasticity or transdifferentiation of adult stem cells has been challenged by the phenomenon of cell fusion. In this work, we examined whether neonatal cardiomyocytes fuse with various somatic cells including endothelial cells, cardiac fibroblasts, bone marrow
Fernando H Biase et al.
iScience, 7, 16-29 (2018-09-30)
We developed the Rainbow-seq technology to trace cell division history and reveal single-cell transcriptomes. With distinct fluorescent protein genes as lineage markers, Rainbow-seq enables each single-cell RNA sequencing (RNA-seq) experiment to simultaneously decode the lineage marker genes and read single-cell
Francesco Trovato et al.
Nature communications, 11(1), 6194-6194 (2020-12-05)
Genetic mosaicism, a condition in which an organ includes cells with different genotypes, is frequently present in monogenic diseases of the central nervous system caused by the random inactivation of the X-chromosome, in the case of X-linked pathologies, or by

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