C6992
Anti-CENP-A (C-terminal) antibody produced in rabbit
~2 mg/mL, affinity isolated antibody, buffered aqueous solution
Sinónimos:
Anti-CenH3, Anti-Centromere Protein A
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About This Item
Productos recomendados
origen biológico
rabbit
conjugado
unconjugated
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
formulario
buffered aqueous solution
mol peso
antigen 17 kDa
reactividad de especies
human
concentración
~2 mg/mL
técnicas
western blot: 1-2 μg/mL using HEK-293 cells expressing human CENP-A
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... CENPA(1058)
Descripción general
Centromere protein A is a protein encoded by the CENPA gene in humans. It is a histone H3 variant that has centromere-specific nucleosomal property and is located at neocentromere found on chromosome 10. CENP-A- binding clusters is associated with nucleosomal blocks within the neocentromeric chromatin.
Inmunógeno
synthetic peptide corresponding to amino acids 124-140 located at the C-terminus of human CENP-A, conjugated to KLH. This sequence is highly conserved (88% identity) in bovine and dog CENP-A, and shows 60% identity with histone H3.
Aplicación
Anti-CENP-A (C-terminal) antibody produced in rabbit is suitable for western blotting at a concentration of 1-2μg/mL using HEK-293 cells expressing human CENP-A.
Acciones bioquímicas o fisiológicas
The location of the centromere on the chromosome is dependent on the histone H3 variant centromere protein A (CENP-A). The N-terminal tail of CENP-A is highly different from other H3 variants. Serine 7 of CENP-A plays an important role in mitosis-specific phosphorylation. It also plays a key role in kinetochore assembly. Alteration in CENP-A causes various human cancers. CENP-A may play an important role in epithelial ovarian cancer (EOC) and may serve as prognostic marker. It acts as a potential target for gene therapy in the treatment of EOC. The NH2 terminus of human CENP-A is essential for mitotic progression and necessary for the platform function of CENP-A centromeric chromatin in the assembly as well as maintenance of active kinetochores.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Jun-Jun Qiu et al.
Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine, 34(5), 2971-2975 (2013-05-29)
Altered expression of centromere protein-A (CENP-A) is observed in various types of human cancers. However, the clinical significance and pathological role of CENP-A in epithelial ovarian cancer (EOC) remains unclear. The main objective of this investigation was to clarify the
R-M Liu et al.
Folia biologica, 59(3), 105-109 (2013-07-31)
Serine 7 of centromere protein A (CENP-A) is a very important mitosis-specific phosphorylation site. In this study, we demonstrate the subcellular distribution of Ser7 phosphorylated CENP-A during mitosis in MCF-7 cells. The Ser7 phosphorylation of CENP-A was observed beginning at
Aaron O Bailey et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(29), 11827-11832 (2013-07-03)
Centromeres are chromosomal loci required for accurate segregation of sister chromatids during mitosis. The location of the centromere on the chromosome is not dependent on DNA sequence, but rather it is epigenetically specified by the histone H3 variant centromere protein
Anderly C Chueh et al.
Human molecular genetics, 14(1), 85-93 (2004-11-13)
Human neocentromeres are fully functional centromeres that arise epigenetically from non-centromeric precursor sequences that are devoid of alpha-satellite DNA. Using chromatin immunoprecipitation (ChIP) and BAC-array analysis, we have previously described a 330 kb binding domain for CENP-A (a histone H3
Hernan Diego Folco et al.
Science (New York, N.Y.), 319(5859), 94-97 (2008-01-05)
Heterochromatin is defined by distinct posttranslational modifications on histones, such as methylation of histone H3 at lysine 9 (H3K9), which allows heterochromatin protein 1 (HP1)-related chromodomain proteins to bind. Heterochromatin is frequently found near CENP-A chromatin, which is the key
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