C6788

4-Chloro-1-naphthol

peroxidase substrate, tablet, suitable for use in immunoblotting procedures

• Sinónimos: 4-CN, 4-chloronaphthalen-1-ol
• Fórmula lineal: ClC₁₀H₆OH
• Número de CAS: 604-44-4
• Peso molecular: 178.61
• Beilstein: 509750
• Número CE: 210-068-5
• Número MDL: MFCD00003974
• Código UNSPSC: 12352204
• ID de la sustancia en PubChem: 57654035
• NACRES: NA.83

Propiedades

• Nombre del producto: 4-Chloro-1-naphthol, tablet
• Formulario: tablet
• Nivel de calidad: 200
• mp: 118-121 °C (lit.)
• solubilidad: methanol: 10 mL, clear, colorless
• temp. de almacenamiento: −20°C
• cadena SMILES: Oc1ccc(Cl)c2ccccc12
• InChI: 1S/C10H7ClO/c11-9-5-6-10(12)8-4-2-1-3-7(8)9/h1-6,12H
• Clave InChI: LVSPDZAGCBEQAV-UHFFFAOYSA-N

Descripción

Especificidad

4-Chloro-1-Naphthol is a peroxidase substrate suitable for use in immunoblotting procedures. This substrate produces an insoluble end product that is blue in color and can be observed visually.

Aplicación

4-Chloro-1-naphthol has been used as a substrate in enzyme-linked immunospot (ELISpot) assay for detection of the spots. It has also been used as a precipitating chromogenic substrate for visualizing the antigen−antibody-reactive bands following western blotting.

Cantidad

Contains 30 mg substrate per tablet.

Reconstitución

Dissolve 1 tablet in 10 ml of methanol. Add 2 ml of methanol stock solution to 10 ml of triethanolamine buffered saline, pH 7.5. Add 5 μl of fresh 30% hydrogen peroxide (Sigma Product No. H-1009) immediately prior to use.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves