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Merck
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Key Documents

C3367

Sigma-Aldrich

Anti-CIITA antibody, Mouse monoclonal

clone 7-1H, purified from hybridoma cell culture

Sinónimos:

Anti-C2TA, Anti-MHC Class II Transactivator

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About This Item

Número MDL:
MFCD01864254
Código UNSPSC:
12352203
NACRES:
NA.41

origen biológico

mouse

conjugado

unconjugated

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

7-1H, monoclonal

formulario

buffered aqueous solution

mol peso

antigen ~130 kDa

reactividad de especies

mouse, human

concentración

~2 mg/mL

técnicas

immunocytochemistry: suitable
western blot: 2-4 μg/mL using total cell extract of Raji cells

isotipo

IgG1

Nº de acceso UniProt

P33076

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

human ... CIITA(4261)
mouse ... Ciita(12265)

Descripción general

Class II transactivator protein (CIITA) protein contains an acidic region in its N-terminal with transactivation activity, that binds to proteins in the basal transcription machinery and to cAMP response element-binding protein (CREB)-binding protein (CBP). The protein contains motifs for nuclear translocation, GTP binding motif and C-terminal leucine-rich repeats. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ .
Monoclonal Anti-CIITA (mouse IgG1 isotype) is derived from the hybridoma 7-1H produced by the fusion of mouse myeloma cells (Sp20 cells) and splenocytes from BALB/c mice immunized with purified N-terminal recombinant CIITA, amino acids 1-350. CIITA is expressed constitutively in B lymphocytes and in immature dendritic cells, while in other cells its expression is induced by IFN-γ.

Monoclonal Anti-CIITA recognizes human1 and mouse † CIITA.

Aplicación

The antibody may be used in immunoblotting (∼130 kDa) and immunocytochemistry.

Acciones bioquímicas o fisiológicas

Class II transactivator protein (CIITA) interacts with specific transcription factors and creates the appropriate transcriptional scaffold required for HLA-D gene expression.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Giovanna Barbieri et al.
International immunology, 14(8), 839-848 (2002-07-31)
The class II transactivator (CIITA) controls both the constitutive and IFN-gamma inducible expression of HLA-D genes. In addition, through the squelching of another transactivator CREB-binding protein, CIITA was more recently shown to have a wider cellular function, including cell cycle
H Zhou et al.
Journal of immunology (Baltimore, Md. : 1950), 158(10), 4741-4749 (1997-05-15)
The MHC class II transactivator gene (CIITA) coordinately controls the expression of the three major human class II genes, HLA-DR, HLA-DQ, and HLA-DP. Indeed, patients with one form of MHC class II immunodeficiency disease, due to defective CIITA genes, lack
Isoforms of the class II transactivator protein
Barbieri G, et al.
International Immunology, 14(8), 839-848 (2002)
W Wiszniewski et al.
Journal of immunology (Baltimore, Md. : 1950), 167(3), 1787-1794 (2001-07-24)
The expression of MHC class II molecules is essential for all Ag-dependent immune functions and is regulated at the transcriptional level. Four trans-acting proteins control the coordinate expression of MHC class II molecules: class II trans-activator (CIITA), regulatory factor binding
Rodrigo Naves et al.
International immunology, 14(5), 481-491 (2002-04-30)
MHC class II expression defects have been evidenced in several human tumor cell lines originating from lung cancers or retinoblastoma. Accordingly, the mouse adenocarcinoma and fibrosarcoma cell lines, RAG and L(tk-), do not express I-A and I-E molecules even when
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