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Key Documents

C2724

Sigma-Aldrich

Anti-Calbindin-D-28K (EG-20) antibody produced in rabbit

enhanced validation

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Anti-CALB, Anti-D-28K

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.44

origen biológico

rabbit

conjugado

unconjugated

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

primary antibodies

clon

polyclonal

formulario

buffered aqueous solution

mol peso

antigen 28 kDa

reactividad de especies

rat

validación mejorada

independent
Learn more about Antibody Enhanced Validation

técnicas

immunohistochemistry: 1:2,000 using sections of rat cerebellum
western blot: 1:2,000 using a whole extract of rat brain

Nº de acceso UniProt

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

Información sobre el gen

Descripción general

Calbindin-D-28K (CALB1) is a calcium binding protein in the brain. This 27kDa protein is located on human chromosome 8q21.
Calbindin-D-28k is an intracellular protein characterized by EF-hand type structural motifs that bind to Ca2+. This protein modulates synaptic plasticity and signal transduction . Anti-Calbindin-D-28K (EG-20) antibody recognizes rat calbindin-D-28K (28 kDa). Due to interspecies similarity, reactivity with human, mouse, bovine, chicken, porcine, and frog is expected, but has not been confirmed. Staining of calbindin-D-28K is specifically inhibited with the calbindin-D-28K immunizing peptide by immunoblotting.

Inmunógeno

The immunogen sequence is specific for calbindin-D-28K and is not found in other members of the EF-hand family such as calbindin-D-9K, calretinin, myosin light chain, parvalbumin, S-100a, S-100b, S100A2 (S100L) and S100A6 (calcyclin). This sequence is highly conserved across species, including human, mouse, bovine, chicken, porcine, and frog calbindin-D-28K sequences.
synthetic peptide corresponding to the C-terminal region of rat calbindin-D-28K (amino acids 225-244 with N-terminally added Lys-Gly) conjugated to KLH.

Aplicación

Anti-Calbindin-D-28K (EG-20) antibody is suitable for use in immunohistochemistry (1:2,000 using sections of rat cerebellum), immunoblot and western blot (1:2,000 using a whole extract of rat brain). Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min., at RT or 37 ° C) improves immunohistochemical staining with the antibody.
Anti-Calbindin-D-28K (EG-20) antibody has been used in immunohistochemical (IHC) peroxidase staining and cupric silver staining. It has also been used in immunofluorescence.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Nota de análisis

Enzymatic predigestion of formalin-fixed, paraffin-embedded sections by proteolytic enzymes (e.g., 0.1% trypsin or protease, 10 min at RT or 37 °C) improves immunohistochemical staining.

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Opcional

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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