C-26030

Renal Epithelial Cell Growth Medium 2

Ready-to-use kit including Basal Medium and SupplementMix, 500 ml

• Sinónimos: Epithelial Growth Medium, Growth Medium for Epithelial Cells, Renal Cell Medium
• Código UNSPSC: 12352207
• NACRES: NA.71

Propiedades

• formulario: liquid
• envase: pkg of 500 ml
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: wet ice

Descripción

Descripción general

Renal Epithelial Cell Growth Medium 2 (Ready-to-use)

Aplicación

Renal Epithelial Cell Growth Medium 2 is an optimized low-serum (5% V/V) medium that additionally contains Epinephrine. The combination of low serum and selected growth factors ensures successful growth in vitro making the Renal Epithelial Cell Growth Medium 2 the first choice for PromoCell Renal Epithelial Cells. The Renal Epithelial Cell Growth Medium 2 is optimized for primary human cells, but can also be used for porcine and mouse renal epithelial cells.Recommended for:Human Renal Epithelial Cells (HREpC)Human Renal Cortical Epithelial Cells (HRCEpC)

Componentes

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium Kit: Includes Basal Medium and SupplementPackSupplementMix: Contains all media supplements premixed in one vialSupplementPack: Contains all media supplements as individual vials

Calidad

All lots of PromoCell Renal Epithelial Cell Media are subjected to comprehensive quality control tests using primary human renal epithelial cells. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested renal epithelial cells. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Nota de preparación

Final supplement concentrations after addition to the basal medium:Fetal Calf Serum: 0.05 ml / ml Epidermal Growth Factor (recombinant human): 10 ng / mlInsulin (recombinant human): 5 µg / mlHydrocortisone: 36 ng / mlEpinephrine: 0.5 µg / mlTriiodo-L-thyronine: 4 pg / mlTransferrin (recombinant human): 5 µg / ml

Almacenamiento y estabilidad

Store the Basal Medium at 4 to 8°C in the dark and the SupplementMix or SupplementPack at -20°C immediately after arrival. Do not freeze the Basal Medium. If stored properly, the products are stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C.

Rutina de subcultivo

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Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids