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Key Documents

C-24405

Sigma-Aldrich

Melanocyte Growth Medium M2

Basal Medium, phenol red-free, 500 ml

Sinónimos:

Recommended with C-39420

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About This Item

Código UNSPSC:
12352207
NACRES:
NA.81

formulario

aqueous solution

envase

pkg of 500 ml

técnicas

cell culture | mammalian: suitable

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

Melanocyte M2 Basal Medium, phenol red-free (supplements sold separately)

To form a complete medium, it should be combined with the media supplements: C-39420

Aplicación

Melanocyte Growth Medium M2 is a serum-free and PMA (Phorbol Myristate Acetate)-free Medium that was developed for the optimized in vitro cultivation of human melanocytes. It is also suitable for human pigment epithelial cells, neuronal cells, and melanoma cells. The medium is optimized for primary human cells, but can also be used for equine, porcine, mouse, and rat melanocytes.Recommended for:Normal Human Epidermal Melanocytes (NHEM), juvenile foreskin, cultured in M2 MediumNormal Human Epidermal Melanocytes (NHEM), adult donor, cultured in M2 Medium

Componentes

Part of a Cell Culture System offered in a variety of formats:Growth Medium (Ready-to-use): Includes Basal Medium and SupplementMixGrowth Medium (Ready-to-use): Includes phenol red-free Basal Medium and SupplementMixSupplementMix: Contains all media supplements premixed in one vial

Calidad

All lots of PromoCell Melanocyte Growth Medium M2 are subjected to comprehensive quality control tests using primary human melanocytes. Each lot is checked for growth promoting activity, adherence rate, and typical morphology of the tested melanocytes. Approved in-house lots of media are used as a reference. In addition, all lots of media have been tested for the absence of microbial contaminants (fungi, bacteria, mycoplasma).

Almacenamiento y estabilidad

Store the Basal Medium at 4 to 8°C in the dark immediately after arrival. Do not freeze the Basal Medium. If stored properly, the medium is stable until the expiry date stated on the label. After adding the supplements to the Basal Medium, the shelf life of the complete medium is 6 weeks at 4 to 8°C. Do not freeze the complete medium. For use, pre-warm only an aliquot of the complete medium and keep the remaining medium refrigerated at 4 to 8°C

Rutina de subcultivo

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Opcional

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Artículos

Primary Human Epidermal Melanocytes and growth media optimized for in vitro cultivation of human melanocytes, human pigment epithelial cells, melanoma cells, and neuronal cells. Protocols for handling, cell passaging, media, and product use.

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