C1427

Cytochrome b₅ human

recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

• Sinónimos: Cytochrome b5
• Número MDL: MFCD02687283
• Código UNSPSC: 12161501
• NACRES: NA.47

Propiedades

• origen biológico: human
• Nivel de calidad: 200
• recombinante: expressed in E. coli (histidine-tagged)
• Análisis: ≥90% (SDS-PAGE)
• formulario: solution
• mol peso: 16 kDa
• técnicas: inhibition assay: suitable
• idoneidad: suitable for molecular biology
• Nº de acceso UniProt: P00167
• Condiciones de envío: dry ice
• temp. de almacenamiento: −70°C
• Información sobre el gen: human ... CYB5A(1528)

Descripción

Descripción general

Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.

Acciones bioquímicas o fisiológicas

Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.

Cytochrome b₅ is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.

Forma física

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

Nota de análisis

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM^-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b₅.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)