C-14029

Human Cardiac Microvascular Endothelial Cells (HCMEC)

1 million cells per pellet

• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• origen biológico: human heart (ventricles)
• envase: pkg of 1,000,000 cells
• morfología: (endothelial)
• aplicaciones: cell analysis (DNA, RNA, and protein)
• enfermedades relevantes: diabetes; hypertension; ischemia/reperfusion injury
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C

Descripción

Descripción general

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details. Cells are dissolved in RNAlater for subsequent RNA, DNA, or protein analysis. Note that cell pellets cannot be revived.

Origen línea celular

Heart

Aplicación

Primary Human Cardiac Microvascular Endothelial Cells (HCMEC) are isolated from heart ventricles from a single donor. The cells are routinely analyzed by immunofluorescent staining: they stain positive for CD31 and Willebrand factor and negative for smooth muscle alpha-actin.HCMEC intensively interact with cardiomyocytes and therefore have a characteristic phenotype different from other microvascular endothelial cells. HCMEC play an important role in the physiological regulation of coronary blood flow and capillary exchange. Medical conditions such as hypertrophy, ischemia, hypertension, and diabetes mellitus are associated with endothelial dysfunction.

Calidad

Rigid quality control tests are performed for each lot of the corresponding cells. They are tested for cell viability, cell morphology, growth performance and for cell-type specific markers. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertencia

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Forma física

Pellet is dissolved in RNAlater^® for downstream analysis of DNA, RNA or protein profiles. Cells are not revivable.

Rutina de subcultivo

Click here for more information.

Otras notas

The Cell Pellets can be stored indefinitely at -20°C, and for up to 1 month at 4°C. Note: The Cell Pellets will not freeze at -20°C. Always centrifuge product briefly before opening vial.

Información legal

RNAlater is a registered trademark of Ambion, Inc.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable