C1235
Cholesterol Oxidase microbial
recombinant, lyophilized powder, ≥10 units/mg protein
Sinónimos:
Cholesterol: oxygen oxidoreductase
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About This Item
Productos recomendados
Nivel de calidad
Formulario
lyophilized powder
actividad específica
≥10 units/mg protein
mol peso
55 kDa
solubilidad
50 mM potassium phosphate buffer, pH 7.0: soluble
temp. de almacenamiento
−20°C
Aplicación
Cholesterol oxidase is used to determine serum cholesterol. The enzyme also finds application in the microanalysis of steroids in food samples and in distinguishing 3-ketosteroids from 3b-hydroxysteroids. Transgenic plants expressing cholesterol oxidase are being investigated in the fight against the cotton boll weevil. CHOD has also been used as a molecular probe to elucidate cellular membrane structures.
Acciones bioquímicas o fisiológicas
Cholesterol oxidase (CHOD) is a monomeric flavoprotein containing FAD that catalyzes the first step in cholesterol catabolism. This bifunctional enzyme oxidizes cholesterol to cholest-5-en-3-one in an FAD-requiring step. This is subsequently isomerized to cholest-4-en-3-one with the release of H2O2. Optimum pH of the enzyme is 7.0. Hg2+, Ag+, ionic detergents inhibit the enzyme activity.
Definición de unidad
One unit will convert 1.0 μmol of cholesterol to 4-cholesten-3-one per minute at 37 °C and pH 7.0 in a peroxidase linked system.
Nota de preparación
Dissolves in cold 50 mM potassium phosphate buffer, pH 7.0. Solution is to be prepared just before use.
Palabra de señalización
Danger
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Resp. Sens. 1
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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The Journal of biological chemistry, 284(34), 22978-22987 (2009-06-13)
A uniquely sensitive method for ceramide domain detection allowed us to study in detail cholesterol-ceramide interactions in lipid bilayers with low (physiological) ceramide concentrations, ranging from low or no cholesterol (a situation similar to intracellular membranes, such as endoplasmic reticulum)
Analytical and bioanalytical chemistry, 390(1), 155-168 (2007-10-03)
The present review draws a general picture of the bioanalytical applications of electro-chemiluminescent reactions (ECL). Only the two main ECL reactions-i.e. the luminol-based and Ru(bpy)(3)(2+)-based reactions-are considered for application in the fields of enzyme biosensors, immunochemical biosensors, DNA biosensors, and
Effect of cholesterol concentration on organization of viral and vesicle membranes. Probed by accessibility to cholesterol oxidase.
The Journal of biological chemistry, 255(12), 5802-5806 (1980-06-25)
Plant physiology, 126(3), 1116-1128 (2001-07-18)
Cholesterol oxidase represents a novel type of insecticidal protein with potent activity against the cotton boll weevil (Anthonomus grandis grandis Boheman). We transformed tobacco (Nicotiana tabacum) plants with the cholesterol oxidase choM gene and expressed cytosolic and chloroplast-targeted versions of
Protein engineering, 15(6), 477-484 (2002-06-26)
Despite the structural similarities between cholesterol oxidase from Streptomyces and that from Brevibacterium, both enzymes exhibit different characteristics, such as catalytic activity, optimum pH and temperature. In attempts to define the molecular basis of differences in catalytic activity or stability
Protocolos
Enzymatic Assay of Cholesterol Oxidase
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