C-12221

Human Coronary Artery Endothelial Cells (HCAEC)

500,000 cryopreserved cells

• Sinónimos: HCAEC cells
• Código UNSPSC: 41106514
• NACRES: NA.81

Propiedades

• origen biológico: human coronary artery
• envase: pkg of 500,000 cells
• morfología: ( endothelial)
• técnicas: cell culture | mammalian: suitable
• Condiciones de envío: dry ice
• temp. de almacenamiento: −196°C

Descripción

Descripción general

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Origen línea celular

Artery

Aplicación

Primary Human Coronary Artery Endothelial Cells (HCAEC) are isolated from the coronary arteries (the right and the left coronary artery, including the anterior descending and the circumflex branches) from a single donor and are provided in a cryopreserved format. They are crucially involved in the regulation of coronary blood flow and cardiac functions and are consequently useful for in vitro studies of diseases such as thrombosis, atherosclerosis, and hypertension. HCAEC are also suitable for studying functional vasodilators, such as prostacyclin, bradykinin, or nitric oxide.

Calidad

Rigid quality control tests are performed for each lot of Endothelial Cells from large vessels. They are tested for cell morphology, adherence rate, and cell viability. Flow cytometric analyses for cell-type specific markers, e.g. von Willebrand Factor (vWF) and CD31, as well as Dil-Ac-LDL uptake assays are also carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertencia

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutina de subcultivo

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Otras notas

Recommended Plating Density: 5000 - 10000 cells per cm²Passage After Thawing: P3Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Productos recomendados

Recommended Primary Cell Culture Media:Link

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Información de seguridad

• Código de clase de almacenamiento: 12 - Non Combustible Liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable