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C-12210

Sigma-Aldrich

Human Dermal Microvascular Endothelial Cells (HDMEC)

Juvenile foreskin, 500,000 cryopreserved cells

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About This Item

Código UNSPSC:
41106514
NACRES:
NA.81

origen biológico

human foreskin (juvenile dermis)

envase

pkg of 500,000 cells

morfología

( endothelial)

técnicas

cell culture | mammalian: suitable

Condiciones de envío

dry ice

temp. de almacenamiento

−196°C

Descripción general

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Origen línea celular

Skin

Aplicación

Primary Human Dermal Microvascular Endothelial Cells (HDMEC) are isolated from the dermis of juvenile foreskin and are provided in a cryopreserved format. Since the dermis contains blood and lymphatic capillaries, HDMEC comprise Blood and Lymphatic Microvascular Endothelial Cells. Both have a common origin and can be identified by several markers.

Calidad

Rigid quality control tests are performed for each lot of Microvascular Endothelial Cells. They are tested for cell morphology and cell type specific markers, e.g. von Willebrand Factor (vWF), CD31 and Podoplanin using flow cytometric analyses. Growth performance is tested through multiple passages up to 15 population doublings (PD) without antibiotics or antimycotics. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Advertencia

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Rutina de subcultivo

Click here for more information.

Otras notas

Recommended Plating Density: 10000 - 20000 cells per cm2Passage After Thawing: P2Tested Markers: von Willebrand Factor (vWF) positive, CD31 positive, Dil-Ac-LDL uptake positiveGuaranteed population doublings: >15

Productos recomendados

Recommended Primary Cell Culture Media:Link

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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