C0730
Anti-CD137 antibody produced in goat
affinity isolated antibody, lyophilized powder
Sinónimos:
Anti-4-1BB
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
origen biológico
goat
conjugado
unconjugated
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
primary antibodies
clon
polyclonal
formulario
lyophilized powder
reactividad de especies
human
técnicas
capture ELISA: 0.2-0.8 μg/mL
flow cytometry: 2.5 μg using 106 human T cells treated with PHA
immunocytochemistry: 5-15 μg/mL
western blot: 0.1 μg/mL
impurezas
<0.10 EU/μg endotoxin
Nº de acceso UniProt
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... TNFRSF9(3604)
Descripción general
CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes.
Inmunógeno
mouse myeloma cell line NS0-derived recombinant human 41BB/TNFRSF9/CD137 Leu24Gln186
Aplicación
Anti-CD137 antibody produced in goat is suitable for the following applications:
- Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
- ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
- Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
- Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
- Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.
Acciones bioquímicas o fisiológicas
CD137 in T lymphocytes helps to regulate activation, proliferation and apoptosis. CD137 and CD28 are co-stimulatory molecules of T cell activation. It plays an important role in regulating T-cell-dependent immune responses. The expression of CD137 correlates negatively with lymphocyte proliferation and induces cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and helps in prolongs survival.
Forma física
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
Reconstitución
Reconstitute at 0.2 mg/mL in sterile PBS
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
J Langstein et al.
Journal of immunology (Baltimore, Md. : 1950), 160(5), 2488-2494 (1998-03-14)
CD137 (ILA/4-1BB), a member of the TNF receptor family, was shown previously to inhibit proliferation and to induce apoptosis in T lymphocytes. In this study, we identify CD137 as a novel and potent monocyte activation factor. CD137 protein induces expression
J Langstein et al.
Journal of leukocyte biology, 65(6), 829-833 (1999-06-25)
CD137 (ILA/4-1BB), a member of the tumor necrosis factor (TNF) receptor family, promotes adherence and prolongs survival of human peripheral monocytes. It induces a strong expression of macrophage colony-stimulating factor (M-CSF), an essential monocyte survival factor. Monocyte survival induced by
G Kienzle et al.
International immunology, 12(1), 73-82 (1999-12-23)
Human CD137 is a member of the tumor necrosis factor (TNF) receptor family and the homologue of murine 4-1BB. Recent studies have demonstrated that CD137 promotes accessory T cell activation, and regulates proliferation and survival of T lymphocytes. This study
J Michel et al.
Cytokine, 12(6), 742-746 (2000-06-14)
CD137 is a member of the tumour necrosis factor receptor family which delivers a potent co-stimulatory signal to T lymphocytes. Soluble forms of CD137 (sCD137) can be found at enhanced levels in sera of patients with rheumatoid arthritis. Here we
J C Hurtado et al.
Journal of immunology (Baltimore, Md. : 1950), 155(7), 3360-3367 (1995-10-01)
The expression of the murine T cell Ag 4-1BB, a member of the TNF-R family, is induced by T cell activation. Previously, we and others had shown that signaling through 4-1BB enhanced proliferative T cell responses. To investigate a potential
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico