B4554

BES

BioPerformance Certified, suitable for cell culture, ≥99.0%

• Sinónimos: N,N-Bis(2-hydroxyethyl)-2-aminoethanesulfonic acid, N,N-Bis(2-hydroxyethyl)taurine
• Fórmula empírica (notación de Hill): C₆H₁₅NO₅S
• Número de CAS: 10191-18-1
• Peso molecular: 213.25
• Beilstein: 1781572
• Número CE: 233-465-5
• Número MDL: MFCD00007533
• Código UNSPSC: 12161700
• ID de la sustancia en PubChem: 24891788
• NACRES: NA.25

Propiedades

• grado: BioPerformance Certified
• Ensayo: ≥99.0%
• Formulario: crystalline
• técnicas: cell culture | mammalian: suitable
• impurezas: endotoxin and bioburden, tested
• pH: 2.5-5.0 (25 °C, 213.3 g/L)
• intervalo de pH útil: 6.4-7.8
• pKa (25 °C): 7.1
• solubilidad: water: 0.5 g/mL, clear, colorless
• aplicaciones: diagnostic assay manufacturing
• actividad extraña: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• temp. de almacenamiento: room temp
• cadena SMILES: OCCN(CCO)CCS(O)(=O)=O
• InChI: 1S/C6H15NO5S/c8-4-1-7(2-5-9)3-6-13(10,11)12/h8-9H,1-6H2,(H,10,11,12)
• Clave InChI: AJTVSSFTXWNIRG-UHFFFAOYSA-N

Descripción

Descripción general

N-N-bis(2-hydroxyethyl)-2-aminoethanesulfonic acid (BES) is a zwitterionic sodium sulphate buffer used in biochemistry and molecular biology research. BES buffer used as a secondary pH standard can help maintain a stable pH range in solutions, particularly in the range of 6.2-7.6 pH range of 6.6 to 7.4. It ​is used in a study to evaluate that buffers minimize the action of interfering factors in the bacterial endotoxins test. The application of BES as a buffer solution for calcium phosphate-mediated transfection of eukaryotic cells with plasmid DNA has also been studied.

Aplicación

BES buffer has been used:

• as a supplement in opti-minimal essential medium (MEM) culture media to culture thymocytes for In vitro analysis of thymocyte apoptosis
• as a component of borate buffer saline (BBS) for adeno-associated virus (AAV) vector production, extraction, and purification
• as a component of diluent one for semen collection and cryopreservation

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves