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B2787

Sigma-Aldrich

Biotinylated Molecular Weight Marker

mol wt 6,500-180,000 Da

Sinónimos:

protein markers, protein molecular weight markers, protein standards

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About This Item

Código UNSPSC:
12352200
eCl@ss:
34058011
NACRES:
NA.25

formulario

lyophilized powder

Nivel de calidad

200

uso

 vial sufficient for 200 applications (on 10 cm × 10 cm mini gels)

mol peso

6,500-180,000 Da

envase

vial of 0.1 mg protein (also contains 0.1 mg NaCl and 0.1 mg sucrose)

temp. de almacenamiento

−20°C

Aplicación

For SDS-PAGE and protein transfer, the Biotinylated Molecular Weight Standard Mixture contains proteins, which have been biotin conjugated for use as SDS-PAGE standards for the estimation of the molecular weights of blotted proteins. Visualization is accomplished using streptavidin-peroxidase (Cat. No. S2438) and a color development reagent such as 4-Chloro-1-naphthol (Cat. No. C8890), eliminating the need to cut out separate lanes for general protein stains and can be performed simultaneously with immunostaining procedures. These standards may be used with either the Laemmli or the Weber and Osborn SDS-PAGE procedures.

Componentes

This product contains:
  • α2-macroglobulin (equine serum)
  • β-galactosidase (E. coli)
  • phosphorylase b (rabbit muscle)
  • catalase (bovine liver)
  • alcohol dehydrogenase (yeast)
  • carbonic anhydrase (bovine erythrocyte)
  • trypsin inhibitor (soy bean)
  • lysozyme (chicken egg)
  • aprotinin (bovine lung)
Each vial contains approximately 33% protein, 33% NaCl and 33% sucrose.

Otras notas

Contains nine biotinylated proteins (approx 0.1 mg total).

Reconstitución

Each vial is reconstituted with 1 mL of 1x sample buffer (Cat. No. S3401) and incubated for 2-3 minutes at 100 °C.

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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D Della-Penna et al.
Analytical biochemistry, 152(2), 329-332 (1986-02-01)
Protein molecular weight standards were biotinylated by reaction with biotinyl-N-hydroxysuccinimide ester. The biotinylated proteins were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and electrophoretically transferred to nitrocellulose paper. The resolved protein bands were detected by formation of a streptavidin-biotin/horseradish peroxidase
The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
K Weber et al.
The Journal of biological chemistry, 244(16), 4406-4412 (1969-08-25)
Eduardo L V Silveira et al.
Clinical and vaccine immunology : CVI, 15(8), 1292-1300 (2008-06-27)
Earlier studies have demonstrated in A/Sn mice highly susceptible to Chagas' disease protective immunity against lethal Trypanosoma cruzi infection elicited by vaccination with an open reading frame (ORF) expressed by amastigotes. In our experiments, we used this mouse model to
Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
U K Laemmli
Nature, 227(5259), 680-685 (1970-08-15)

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