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Merck

AP0100

Sigma-Aldrich

Alkaline Phosphatase Diethanolamine Activity Kit

sufficient for 100 tests

Sinónimos:

ALP Activity Kit

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About This Item

Código UNSPSC:
12352202
NACRES:
NA.26

uso

sufficient for 100 tests

Nivel de calidad

temp. de almacenamiento

2-8°C

Descripción general

The Alkaline Phosphatase Diethanolamine Detection Kit provides ready-to-use reagents for detecting the presence of alkaline phosphatase activity. This simple assay to detect alkaline phosphatase activity uses p-Nitrophenyl Phosphate (pNPP) as the substrate.

Aplicación

Alkaline Phosphatase Diethanolamine Activity Kit has been used:
  • in the detection of alkaline phosphatase (ALP) levels in the blood serum and osteoblasts
  • for monitoring ALP levels during osteoblast differentiation in mouse myoblast C2C12 cells
  • for determining ALP activity in the bone metastasis model of lung cancer
  • for measuring ALP levels in colon tissue homogenates

Idoneidad

Suitable for the measurement of alkaline phosphatase activity in a variety of samples

Principio

Alkaline phosphatase hydrolyzes pNPP to p-nitrophenol and inorganic phosphate. During incubation of the alkaline phosphatase sample and substrate at 37 C, the reaction is followed by monitoring the increase in absorbance at 405 nm.

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS

  • Alkaline Phosphatase Diethanolamine Reaction Buffer

  • P4744Phosphatase substrate, powderSDS

  • P6774Phosphatase, Alkaline from bovine intestinal mucosa, buffered aqueous solution, ≥2,000 DEA units/mg proteinSDS

Pictogramas

Health hazardCorrosion

Palabra de señalización

Danger

Frases de peligro

Clasificaciones de peligro

Eye Dam. 1 - Repr. 2 - Skin Irrit. 2 - STOT RE 2 Oral

Órganos de actuación

Kidney,Liver,Blood

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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