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Merck

A9205

Sigma-Aldrich

Seroalbúmina bovina solution

30% in saline, fatty acid free, aseptically filled

Sinónimos:

Albúmina solution from bovine serum, Disolución BSA

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About This Item

Número de CAS:
Número MDL:
Código UNSPSC:
12352202
NACRES:
NA.27

origen biológico

bovine

Nivel de calidad

esterilidad

aseptically filled

Análisis

28-32% protein basis (biuret)

formulario

solution

purificado por

cold ethanol fractionation

no contiene

stabilizer or preservative

origen

USA origin

concentración

28-32% protein (biuret)
30% in saline

free fatty acid content

≤0.01%

técnicas

ELISA: suitable
electrophoresis: suitable
western blot: suitable

impurezas

≤0.01% free fatty acid

Nº de acceso UniProt

actividad extraña

BT virus, none detected
VSV virus, none detected

temp. de almacenamiento

2-8°C

Información sobre el gen

bovine ... ALB(280717)

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Descripción general

Bovine Serum Albumin (BSA) has three homologous domains, each subdivided it two domain (A and B). It is a α-helical globular protein and shares structural features with human serum albumin.

Aplicación

Bovine Serum Albumin solution has been used:
  • as Dulbecco′s modified Eagle′s medium supplement for culturing HepG2 human liver cancer cells
  • as a component of hepatocyte recovery medium
  • as a component of fusion and activation buffers for in vitro reconstructed sheep embryos

Acciones bioquímicas o fisiológicas

Bovine Serum Albumin (BSA) is the major protein present in blood and has calcium binding sites and regulates metal homeostasis. It is a crucial component of the cell culture medium and promotes embryonic stem cells (hESC) differentiation. Due to its high solubility, low cost and purity it is widely used in cosmetic and pharmaceutical industry. BSA is used as a blocking agent used in enzyme linked immunosorbent assay (ELISA) for preventing non-specific binding of antigens and antibodies to the microtiter plates.

Nota de preparación

Initial fractionation by cold alcohol precipitation

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable

Equipo de protección personal

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Lipotoxicity is associated with a high level of fatty acid accumulation in pancreatic β-cells. An overload of free fatty acids contributes to pancreatic β-cell apoptosis and dysfunction. Insulin secretion involves sequential ionic events upon glucose stimulation. ATP sensitive potassium (KATP)
Down-regulation of the mitochondrial 3-hydroxy-3-methylglutaryl-CoA synthase gene by insulin: the role of the forkhead transcription factor FKHRL1
Nadal A, et al.
The Biochemical Journal, 366(1), 289-297 (2002)

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