A8706
Avidin from egg white
recombinant, expressed in corn, ≥12 units/mg protein (E1%/280)
Sinónimos:
AVID_CHICK
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About This Item
Productos recomendados
recombinante
expressed in corn
Nivel de calidad
Ensayo
≥80% protein basis (biuret)
Formulario
powder
actividad específica
≥12 units/mg protein (E1%/280)
mol peso
glycoprotein 66 kDa
subunit 16 kDa
técnicas
ELISA: suitable
immunoblotting: suitable
immunoelectrophoresis: suitable
Nº de acceso UniProt
temp. de almacenamiento
2-8°C
Información sobre el gen
chicken ... AVD(396260)
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Descripción general
Avidin is a homotetrameric glycoprotein found in the egg white of birds, reptiles and amphibians. Each subunit is 16 kDa, singly glycosylated and binds to a molecule of biotin with greater affinity and specificity. Recombinant avidin from corn is similar to avidin from egg white in terms of properties like isoelectric point (pI) and antigenic properties except for the molecular weight. Avidin from corn has low molecular weight than chicken egg-derived avidin. This might be due to less complex glycosylation composition in plants. Commercial production of avidin from corn has certain advantages in terms of availability of greater biomass and avoiding the co-purification of animal virus.
Aplicación
Avidin egg white was used in an assay using functionalized xenon as a biosensor to detect biotin-avidin binding. Egg white was used at 80 nmol.
Avidin forms an extremely strong complex with d-biotin (Kd ~ 10-15). This activity has made both avidin and biotin extremely useful labels in immunochemical methods of detection and quantitation.
Definición de unidad
One unit will bind 1.0 μg of d-biotin.
Nota de preparación
Affinity purified
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Equipo de protección personal
Eyeshields, Gloves, type N95 (US)
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