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Merck

A4914

Sigma-Aldrich

IgG anti-conejo (molécula completa)-Peroxidasa antibody produced in goat

affinity isolated antibody, buffered aqueous solution

Sinónimos:

Goat Anti-Rabbit IgG (whole molecule)–HRP

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About This Item

Número MDL:
Código UNSPSC:
12352203
NACRES:
NA.46

origen biológico

goat

Nivel de calidad

conjugado

peroxidase conjugate

forma del anticuerpo

affinity isolated antibody

tipo de anticuerpo

secondary antibodies

clon

polyclonal

Formulario

buffered aqueous solution

técnicas

direct ELISA: 1:10,000

aplicaciones

research pathology

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

modificación del objetivo postraduccional

unmodified

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Descripción general

IgGs are glycoprotein antibodies that modulate several immune responses. Rabbit polyclonal IgGs against target proteins are often used as primary antibodies in various research applications. Thus, secondary polyclonal anti-rabbit IgGs conjugated to a detectable substrate are useful tools for the immunological analysis of target proteins. Anti-Rabbit IgG (whole molecule)-Peroxidase antibody is specific for all rabbit IgGs and does not bind to human serum proteins in ELISA.

Inmunógeno

Purified rabbit IgG

Aplicación

Se realizaron análisis de inmunoprecipitación y inmunoelectrotransferencia (técnica western) de los lisados celulares C33A utilizando IgG anti-conejo de cabra conjugada con HRP como el anticuerpo secundario.
Anti-Rabbit IgG (whole molecule)-Peroxidase antibody can be used in sandwich ELISA (diluted 1:3000 in PBS-0.05% Tween 20) and immunoelectrophoresis. The product can also be used for western blotting applications (at 1:2000 dilutions in mouse liver homogenates , or in mouse embryonic fibroblasts ).

Otras notas

Antibody adsorbed with human serum proteins.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4 containing 1% bovine serum albumin with preservative.

Nota de preparación

Prepared using the periodate method described by Wilson, M.B., and Nakane, P.K., in Immunofluorescence and Related Staining Techniques, Elsevier/North Holland Biomedical Press, Amsterdam, p215 (1978).

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Pictogramas

Exclamation markEnvironment

Palabra de señalización

Warning

Frases de peligro

Clasificaciones de peligro

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Código de clase de almacenamiento

12 - Non Combustible Liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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