A3682
Anti-Mouse IgG (Fab specific)–Peroxidase antibody produced in goat
affinity isolated antibody, buffered aqueous solution
Sinónimos:
Goat Anti-Mouse IgG (Fab specific)- HRP
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About This Item
Productos recomendados
origen biológico
goat
conjugado
peroxidase conjugate
forma del anticuerpo
affinity isolated antibody
tipo de anticuerpo
secondary antibodies
clon
polyclonal
formulario
buffered aqueous solution
reactividad de especies
mouse
no debe reaccionar con
human, rat
técnicas
direct ELISA: 1:40,000
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150
western blot: 1:80,000-160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg per lane)
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
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Descripción general
Immunoglobulin G (IgG) belongs to the immunoglobulin family and is a widely expressed serum antibody. An immunoglobulin has two heavy chains and two light chains connected by a disulfide bond. It mainly helps in immune defense. It is a glycoprotein. IgG is a major class of immunoglobulin. Mice possess five classes of immunoglobulins- IgM, IgG, IgA, IgD and IgE. Mouse IgG is further divided into five classes- IgG1, IgG2a, IgG2b and IgG3.
Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases. Mouse IgGs have four distinct isotypes, namely, IgG1, IgG2a, IgG2b, and IgG3. IgG1 regulates complement fixation in mice.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is specific for mouse IgG and the Fab fragment of mouse IgG. The antibody reacts with all mouse IgG subclasses (G1, G2a, G2b and G3) and also with mouse IgA, IgM and IgE. Moreover, the antibody conjugate shows no reactivity with the Fc fragment of mouse IgG or with human and rat IgG.
Goat anti-mouse IgG (Fab specific)-peroxidase antibody is specific for mouse IgG and the Fab fragment of mouse IgG. The antibody reacts with all mouse IgG subclasses (G1, G2a, G2b and G3) and also with mouse IgA, IgM and IgE. Moreover, the antibody conjugate shows no reactivity with the Fc fragment of mouse IgG or with human and rat IgG.
Inmunógeno
purified mouse IgG, Fab fragment
Aplicación
Murine IgG levels were quantitated in brain abscess homogenates by ELISA using HRP conjugated goat anti-mouse Fab specific antibody as the detection antibody.
Anti-Mouse IgG (Fab specific)-Peroxidase antibody produced in goat has been used in:
- enzyme-linked immunosorbent assay (ELISA)
- immunohistochemical studies
- dot or immunoblotting
Goat anti-mouse IgG (Fab specific)-peroxidase antibody can be used for western blotting applications. The product can also be used for direct ELISA (1:40,000) and immunohistochemistry (at 1:150, using formalin-fixed, paraffin-embedded sections).
HRP conjugated goat anti-mouse IgG Fab specific was used as the secondary antibody for western blot analysis of bacterial samples of s. equi cells to detect fibrinogen-binding proteins.
Acciones bioquímicas o fisiológicas
Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. IgG2a has cytotropic properties and IgG2 molecules have the ability to activate the complement system. IgG helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.
Forma física
Solution in 0.01 M phosphate buffered saline pH 7.4, containing 0.05% MIT
Nota de preparación
Adsorbed with human IgG and rat serum proteins to reduce background staining with human or rat samples.
Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Palabra de señalización
Warning
Frases de peligro
Consejos de prudencia
Clasificaciones de peligro
Skin Sens. 1
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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