A2917

Acrylamide/Bis-acrylamide

for molecular biology, BioReagent, 19:1 (ratio)

• Sinónimos: Acrylamide/Bisacrylamide, 19:1 (5% crosslinker), Polyacrylamide solution
• Número MDL: MFCD00080848
• Código UNSPSC: 41105319
• ID de la sustancia en PubChem: 329770804
• NACRES: NA.25

Propiedades

• grado: for molecular biology
• Nivel de calidad: 200
• Línea del producto: BioReagent
• Formulario: powder
• cociente alimentación: 19:1 (ratio)
• actividad extraña: DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected
• cadena SMILES: NC(=O)C=C.C=CC(=O)NCNC(=O)C=C
• InChI: 1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)
• Clave InChI: OCQZXMCGTAWGEQ-UHFFFAOYSA-N

Descripción

Descripción general

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Aplicación

Acrylamide/Bis-acrylamide has been used:

• in the preparation of urea polyacrylamide gel during a modified cross-linking affinity purification protocol (cCLAP)
• in microsatellite genotyping and nonfluorescent elastin (ELN) (GT)n polymorphism genotyping
• in polyacrylamide gel for separation of proteins in SDS-PAGE for western blotting

Ready to reconstitute dry powder blends are accurately pre-blended to produce a 40% (w/v) stock solution for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.

Características y beneficios

Eliminates the need to weigh toxic acrylamide and bis-acrylamide.

Reconstitución

Reconstitute by slowly adding the indicated amount of distilled water to the container and dissolve contents.

To make up 100 ml of A2917, slowly add 63 ml distilled or deionized water to the container and dissolve contents. Do not divide the solid. This makes 100 ml of a 40% stock solution.
For the 1 Liter size, use 630 mL of water.

Información de seguridad

• Pictogramas: GHS06,GHS08
• Palabra de señalización: Danger
• Frases de peligro: H301,H312 + H332,H315,H317,H319,H340,H350,H361fd,H372
• Consejos de prudencia: P202 - P280 - P301 + P310 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338
• Clasificaciones de peligro: Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral
• Órganos de actuación: Peripheral nervous system
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges