A2859
Monoclonal Anti-ATG5 antibody produced in mouse
clone ATG5-18, purified immunoglobulin, buffered aqueous solution
Sinónimos:
Anti-APG5, Anti-APG5L, Anti-ASP, Anti-ATG5 autophagy related 5 homolog, Anti-hAPG5
Iniciar sesiónpara Ver la Fijación de precios por contrato y de la organización
About This Item
Productos recomendados
origen biológico
mouse
Nivel de calidad
conjugado
unconjugated
forma del anticuerpo
purified immunoglobulin
tipo de anticuerpo
primary antibodies
clon
ATG5-18, monoclonal
formulario
buffered aqueous solution
mol peso
antigen ~56 kDa
reactividad de especies
human, mouse, rat
concentración
~1.0 mg/mL
técnicas
immunoprecipitation (IP): suitable
western blot: 2.5-5.0 μg/mL using whole extract of human K562 cells
isotipo
IgG2a
Nº de acceso UniProt
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C
modificación del objetivo postraduccional
unmodified
Información sobre el gen
human ... ATG5(9474)
mouse ... Atg5(11793)
rat ... Atg5(365601)
Descripción general
Monoclonal Anti-ATG5 (mouse IgG2a isotype) is derived from the hybridoma ATG5-18 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with human Atg5, conjugated to keyhole limpet hemocyanin (KLH). The autophagy related 5 (ATG5) gene is mapped to human chromosome 6q21 and encodes a 32 kDa protein.
Especificidad
Mouse monoclonal anti-ATG5 antibody reacts with human, rat, and mouse Atg5-Atg12 complex by immunoblotting (∼56kDa) and immunoprecipitation.
Aplicación
Monoclonal Anti-ATG5 antibody produced in mouse has been used in western blotting and immunoprecipitation
Omegasomes were shown to localize to autophagosomes by immunohistochemistry using rabbit anti-ATG5 antibody.
Acciones bioquímicas o fisiológicas
Atg5 (also known as Apg5) is a 32kDa protein that is required for mediating autophagy. Studies have reported that the Atg12-Atg5-Atg16 complex localizes to autophagosome precursors and plays an important role in autophagosome formation. Furthermore, a fragment of Atg5 fragment produced by calpain cleavage is known to have pro-apoptotic properties.
Autophagy related 5 (ATG5), also known as Apg5, is essential for autophagy, a major pathway for bulk degradation of cytoplasmic constituents and organelles. In this process, portions of the cytoplasm are sequestered into double membrane vesicles, the autophagosomes, and subsequently delivered to the lysosome for degradation and recycling. Autophagy is required for normal turnover of cellular components during starvation. It plays an essential role in cellular differentiation, cell death and aging. Defective autophagy may contribute to certain human diseases such as cancer, neurodegenerative diseases, muscular disorders and pathogen infections. Autophagy is an evolutionary conserved pathway seen in all eukaryotic cells.
Forma física
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Cláusula de descargo de responsabilidad
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
¿No encuentra el producto adecuado?
Pruebe nuestro Herramienta de selección de productos.
Opcional
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
10 - Combustible liquids
Clase de riesgo para el agua (WGK)
WGK 2
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
¿Ya tiene este producto?
Encuentre la documentación para los productos que ha comprado recientemente en la Biblioteca de documentos.
Unique role for ATG5 in neutrophil-mediated immunopathology during M. tuberculosis infection
Kimmey JM, et al.
Nature, 528(7583), 565-565 (2015)
Allan Sauvat et al.
EBioMedicine, 30, 261-272 (2018-04-03)
Depending on the length of their carbon backbone and their saturation status, natural fatty acids have rather distinct biological effects. Thus, longevity of model organisms is increased by extra supply of the most abundant natural cis-unsaturated fatty acid, oleic acid
Xian Jiang et al.
Molecular cell, 63(2), 229-239 (2016-07-23)
In response to apoptotic stimuli, mitochondria in mammalian cells release cytochrome c and other apoptogenic proteins, leading to the subsequent activation of caspases and apoptotic cell death. This process is promoted by the pro-apoptotic members of the Bcl-2 family of
Mireia Niso-Santano et al.
The EMBO journal, 34(8), 1025-1041 (2015-01-15)
To obtain mechanistic insights into the cross talk between lipolysis and autophagy, two key metabolic responses to starvation, we screened the autophagy-inducing potential of a panel of fatty acids in human cancer cells. Both saturated and unsaturated fatty acids such
The role of autophagy during the early neonatal starvation period
Kuma A, et al.
Nature, 432(7020), 1032-1032 (2004)
Nuestro equipo de científicos tiene experiencia en todas las áreas de investigación: Ciencias de la vida, Ciencia de los materiales, Síntesis química, Cromatografía, Analítica y muchas otras.
Póngase en contacto con el Servicio técnico