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Merck

A1970

Millipore

Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal

clone P5D4, purified from hybridoma cell culture, PBS suspension

Sinónimos:

Monoclonal Anti-VSV Glycoprotein

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.56

origen biológico

mouse

Nivel de calidad

conjugado

agarose conjugate

forma del anticuerpo

purified immunoglobulin

tipo de anticuerpo

primary antibodies

clon

P5D4, monoclonal

formulario

PBS suspension

clases químicas de analitos

proteins (VSV-G)

técnicas

immunoprecipitation (IP): suitable
protein purification: suitable

isotipo

IgG1

capacidad

≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein)

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

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Descripción general

Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.

Especificidad

The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.

Inmunógeno

synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.

Aplicación

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)
For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.

Forma física

Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Nota de preparación

Prepared using cyanogen bromide-activated agarose.

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Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

nwg

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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