A1106

Anti-ATM antibody,Mouse monoclonal

clone MAT3-4G10/8, purified from hybridoma cell culture

• Sinónimos: Anti-Ataxia-Telangiectasia Mutated
• Número MDL: MFCD01321740
• Código UNSPSC: 12352203
• NACRES: NA.45

Propiedades

• origen biológico: mouse
• Nivel de calidad: 200
• recombinante: expressed in mouse cell line
• conjugado: unconjugated
• forma del anticuerpo: purified from hybridoma cell culture
• tipo de anticuerpo: primary antibodies
• clon: MAT3-4G10/8, monoclonal
• Formulario: PBS solution
• mol peso: antigen ~300 kDa
• reactividad de especies: human, mouse
• envase: antibody small pack of 25 μL
• concentración: ~2 mg/mL
• técnicas: immunoprecipitation (IP): suitable; indirect ELISA: suitable; western blot: 0.1-0.2 μg/mL using HEK-293T total cel extract
• isotipo: IgG1
• Nº de acceso UniProt: Q13315
• Condiciones de envío: dry ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: unmodified
• Información sobre el gen: human ... ATM(472) ; mouse ... Atm(11920)

Descripción

Descripción general

Monoclonal Anti-ATM (mouse IgG1 isotype) is derived from the hybridoma MAT3-4G10/8 produced by the fusion of mouse myeloma cells (NSO) and splenocytes from BALB/c mice immunized with a peptide.

Especificidad

Monoclonal Anti-ATM antibody reacts specifically with mouse and human ATM.

Inmunógeno

peptide spanning positions 1967-1988 of mouse ATM containing a cysteine at its NH2 terminus coupled to KLH.

Aplicación

Monoclonal Anti-ATM antibody can be used in immunoblotting , ELISA and immunoprecipitation.

Acciones bioquímicas o fisiológicas

Anti-Ataxia-Telangiectasia Mutated (ATM) is responsible for the activation and stabilization of p53 in response to double-strand break (DSB). ATM phosphorylates p53 directly on Ser15 and concomitantly activates other kinases that phosphorylate the same molecule on additional sites. Furthermore, human homolog of double minute 2 (Hdm2) is phosphorylated by ATM on Ser395 and this phosphorylation inhibits Hdm2-mediated degradation of p53.

Forma física

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable