316M-1

Perforin (MRQ-23) Mouse Monoclonal Antibody

• Código UNSPSC: 12352203
• NACRES: NA.41

Propiedades

• origen biológico: mouse
• Nivel de calidad: 100; 500
• conjugado: unconjugated
• forma del anticuerpo: culture supernatant
• tipo de anticuerpo: primary antibodies
• clon: MRQ-23, monoclonal
• descripción: For In Vitro Diagnostic Use in Select Regions (See Chart)
• formulario: buffered aqueous solution
• reactividad de especies: human
• envase: vial of 0.1 mL concentrate (316M-14); vial of 0.5 mL concentrate (316M-15); bottle of 1.0 mL predilute (316M-17); vial of 1.0 mL concentrate (316M-16); bottle of 7.0 mL predilute (316M-18)
• fabricante / nombre comercial: Cell Marque^™
• técnicas: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:10-1:50
• isotipo: IgG1
• control: spleen
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: human ... PRF1(5551)

Descripción

Descripción general

Perforin is a pore-forming protein that leads to osmotic lysis of the target cells and subsequently enables granzymes to enter the target cells and activate apoptosis, the cell death program. The expression of perforin is upregulated in activated CD8+ T-cells, but in NK cells the expression is constitutively very high and stable. Perforin expression can also be stimulated in some activated CD4+ T-cells. Although some investigators report a cytolytic potential of CD4+ T-cells, it appears more likely that CD8+ T-cells are the major effector population in Th1-associated inflammatory skin diseases. The role of perforin-mediated cytotoxicity has been demonstrated in various autoimmune diseases. In vitro and in vivo studies suggest that the cytotoxicity of CTLs may be mediated by cytotoxic granules in certain inflammatory diseases in humans. In addition, it seems that T-cell cytotoxicity against keratinocytes is mediated by perforin in some inflammatory skin diseases. Other authors suggest that perforin may have a dual role in alloimmune response (organ transplant applications). In one regard, it has a cytolytic function in acute rejection and, in contrast, it may be responsible for downregulating both CD4- and CD8-mediated alloimmune response.

Calidad

IVD

IVD

IVD

RUO

Ligadura / enlace

Perforin Positive Control Slides, Product No. 316S, are available for immunohistochemistry (formalin-fixed, paraffin-embedded sections).

Forma física

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and <0.1% Sodium Azide

Nota de preparación

Download the IFU specific to your product lot and formatNote: This requires a keycode which can be found on your packaging or product label.

Otras notas

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Información legal

Cell Marque is a trademark of Merck KGaA, Darmstadt, Germany