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3021

Sigma-Aldrich

Transcreener® GDP TR-FRET Assay

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About This Item

Código UNSPSC:
41122100
NACRES:
NA.84

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Descripción general

The Transcreener® GDP TR-FRET Red Assay is a competitive immunoassay for GDP with a far-red, time-resolved Förster-resonance-energy-transfer (TR-FRET) readout. Because it is highly selective for GDP, the assay can be used with any enzyme that converts GTP to GDP, regardless of what other substrates are used. Examples of enzymes include GTPases and fucosyltransferases. The Transcreener® assay is designed specifically for high-throughput screening (HTS), with a single-addition, mix-and-read format. It offers reagent stability and compatibility with commonly used multimode plate readers. The generic nature of the Transcreener® HTS assay platform eliminates delays involved in assay development for new HTS targets and greatly simplifies compound and inhibitor profiling across multiple target families.

The Transcreener® GDP TR-FRET Red Assay provides the following benefits:
  • Accommodates GTP concentrations ranging from 0.1 μM to 1,000 μM.
  • Excellent data quality (Z′ = 0.7) at low substrate conversion (typically 10–30%).
  • Overcomes the need for time-consuming, one-off assay development for individual members within a group transfer enzyme family by using a single set of assay reagents that detect an invariant product.
  • Time-gated detection method largely eliminates interference that can result from prompt fluorescence of test compounds.
  • Far-red tracer further minimizes interference from fluorescent compounds and light scattering.

View full Transcreener® product list

Cantidad

3021-1K = 1,000 assay, 384-well
3021-10K = 10,000 assay, 384-well

Forma física

Kit with buffered aqueous solutions

Información legal

Transcreener is a registered trademark of BellBrook Labs

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Megakaryopoiesis represents a multi-step, often unclear, process leading to commitment, differentiation, and maturation of megakaryocytes (MKs) that release platelets. To identify the novel genes that might help to clarify the molecular mechanisms of megakaryocytopoiesis and be regarded as potential candidates
N Levek-Motola et al.
Human reproduction (Oxford, England), 20(12), 3469-3475 (2005-08-27)
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Journal of the American Society of Nephrology : JASN, 32(2), 357-374 (2021-01-01)
Injury to kidney podocytes often results in chronic glomerular disease and consecutive nephron malfunction. For most glomerular diseases, targeted therapies are lacking. Thus, it is important to identify novel signaling pathways contributing to glomerular disease. Neurotrophic tyrosine kinase receptor 3
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Erythropoietin is a multifunctional cytokine which regulates the number of erythrocytes circulating in mammalian blood. This is crucial in order to maintain an appropriate oxygen supply throughout the body. Stimulation of primary human erythroid progenitors (PEPs) with erythropoietin (Epo) leads

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