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Documentos clave

11012001

FLO-1 cell line human

Sinónimos:

FLO1 Cells

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About This Item

Código UNSPSC:
41106514

origen biológico

human esophagus

Nivel de calidad

modo de crecimiento

Adherent

cariotipo

Not specified

morfología

Epithelial

productos

P53 mutation c.830G>T

receptores

Not specified

técnicas

cell culture | mammalian: suitable

enfermedades relevantes

cancer

Condiciones de envío

dry ice

temp. de almacenamiento

−196°C

Origen línea celular

Distal oesophageal adenocarcinoma

Aplicación

For complete product information, please see ECACC

Perfil del ADN

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 12,13
D5S818: 12,14
D7S820: 8
THO1: 6
TPOX: 9,11
vWA: 16

Medio de cultivo

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

Rutina de subcultivo

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 1-3 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Saturation density approx 105 cells/cm2.

Otras notas

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Cláusula de descargo de responsabilidad

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 3

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Mathieu Francois Derouet et al.
PloS one, 9(12), e115589-e115589 (2015-01-01)
Carcinoma of the esophagus has a high case fatality ratio and is now the 6th most common cause of cancer deaths in the world. We previously conducted a study to profile the expression of miRNAs in esophageal adenocarcinoma (EAC) pre
S J Hughes et al.
Cancer research, 57(24), 5571-5578 (1998-01-04)
This study describes Fas (CD95) expression in Barrett's esophagus, adenocarcinomas of the esophagus, and three esophageal adenocarcinoma cell lines. Immunohistochemical analysis of Barrett's esophagus demonstrated cell surface expression of Fas protein. In contrast, 30.5% of esophageal adenocarcinomas examined by immunohistochemical
Sarah Derks et al.
Cancer immunology research, 3(10), 1123-1129 (2015-06-18)
Esophageal adenocarcinoma is an increasingly common disease with a dismal 5-year survival rate of 10% to 15%. In the first systematic evaluation of the PD-1 pathway in esophageal adenocarcinoma, we identify expression of PD-L2 in cancer cells in 51.7% of
Jurjen J Boonstra et al.
Journal of the National Cancer Institute, 102(4), 271-274 (2010-01-16)
For decades, hundreds of different human tumor type-specific cell lines have been used in experimental cancer research as models for their respective tumors. The veracity of experimental results for a specific tumor type relies on the correct derivation of the

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