106-05A
Human Dermal Fibroblasts: HDF, adult
Sinónimos:
HDF cells
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About This Item
Código UNSPSC:
41106514
NACRES:
NA.81
Productos recomendados
origen biológico
human neonatal foreskin or adult skin (normal)
Nivel de calidad
envase
pkg of 500,000 cells
fabricante / nombre comercial
Cell Applications, Inc
modo de crecimiento
Adherent
cariotipo
2n = 46
morfología
Fibroblast
técnicas
cell culture | mammalian: suitable
Condiciones de envío
dry ice
temp. de almacenamiento
−196°C
Descripción general
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Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)
Dermal Fibroblasts are responsible for producing the extracellular matrix forming the connective tissue of the skin, and play a crucial role during wound healing. HDF provide an excellent model system to study many aspects of cell physiology, and have been utilized in dozens of research publications, particularly those related to skin biology and reprogramming/induced pluripotency studies. HDF were used to create, along with human synovyocytes, induced pluripotent stem cells (iPSC) by using the now classic “Yamanaka cocktail” of Oct3/4, Sox2, Klf4, and c-Myc, the discovery for which Dr. Shinya Yamanaka was awarded the Nobel Prize in 2012 (Takanashi, 2007; 2009), and to characterize iPSC general properties, miRNA expression profiles and differentiation potential (Hayashi, 2010; Ohta, 2011; Koyanagi-Aoi, 2013; Noguchi, 20130; Razak, 2013; Sakurai, 2013; Tanaka, 2013; Yanagida, 2013)
Origen línea celular
Skin
Aplicación
injury recovery, connective tissue research, laminin and fibronectin production, extracellular matrix, tissue organization, wound healing, fibroblasts growth factor response
Componentes
Fibroblast Basal Medium containing 10% FBS & 10% DMSO.
Nota de preparación
- Primary culture, >500,000 cells in Fibroblast Basal Medium containing 10% FBS & 10% DMSO.
- Can be cultured at least 16 doublings.
Medio de cultivo
Fibroblast Growth Medium (116-500) all-in-one ready to use media
Rutina de subcultivo
Please refer to the HDF Culture Protocol.
Cláusula de descargo de responsabilidad
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
obligatorio pero no suministrado
Referencia del producto
Descripción
Precios
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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