W0625

Wright stain

≤4% purity (HPLC), powder

• Sinónimos: Eosin Methylene blue according to Wright
• Número de CAS: 68988-92-1
• Número MDL: MFCD00082143
• Código UNSPSC: 12171500
• NACRES: NA.47

Propiedades

• Nombre del producto: Wright stain, suitable for blood stain
• Ensayo: ≤4% (HPLC)
• Nivel de calidad: 200
• Formulario: powder
• concentración: ≤4% (HPLC)
• técnicas: microbe id | staining: suitable
• color: dark green
• solubilidad: methanol: 10 mg/10 mL
• idoneidad: suitable for blood stain
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: [s]5c6c(nc7c5cc(cc7)N(C)C)cc[c](c6)=[N+](C)C.Brc1c2[o+]c3c(c(c2cc(c1O)Br)c4c(cccc4)C(=O)O)cc(c(c3Br)O)Br
• InChI: 1S/C20H8Br4O5.C16H18N3S/c21-11-5-9-13(7-3-1-2-4-8(7)20(27)28)10-6-12(22)17(26)15(24)19(10)29-18(9)14(23)16(11)25;1-18(2)11-5-7-13-15(9-11)20-16-10-12(19(3)4)6-8-14(16)17-13/h1-6H,(H2-,25,26,27,28);5-10H,1-4H3/q;+1/p+1
• Clave InChI: AXIKDPDWFVPGOD-UHFFFAOYSA-O

Descripción

Descripción general

Wright′s Stain solution is commercially available and is generally used in hematology. Its preparation involves heating methylene blue with sodium bicarbonate and combining the mixture with eosin. It is alkaline, hence, its concentration is continuously changing due to the progressive oxidizing action of the alkali. Therefore, Giemsa′s stain is added to preserve the azurophilic properties of polychrome dyes.

Aplicación

Wright′s Stain is mainly used for the staining of peripheral blood smears and bone marrow smears.

• It is also used for G-banding and helps in the analysis of poorly spread metaphases.
• It has been used to study the cytogenetics of human hematopoietic cells.
• It has also been employed in the karyotyping of human embryonic stem cells.
• It is also used in the staining of chromosomes.
• It is used for the exudate analysis of white blood cell concentration.

Reported to be superior to May-Grunwald Stain in blood staining.

Características y beneficios

The purified dyes in Wright′s Stain eliminate inconsistent staining and yield reproducible lot-to-lot chromogenic responses.

Nota de preparación

Prepared using polychromed (oxidized) methylene blue and eosin Y.

Información de seguridad

• Pictogramas: GHS07
• Palabra de señalización: Warning
• Frases de peligro: H302,H319
• Consejos de prudencia: P280 - P301 + P312 + P330 - P305 + P351 + P338 - P337 + P313
• Clasificaciones de peligro: Acute Tox. 4 Oral - Eye Irrit. 2
• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves