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Merck

P7170

Sigma-Aldrich

Ponceau S solution

BioReagent, 0.1 % (w/v) in 5% acetic acid

Sinónimos:

Ponceau S

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About This Item

Fórmula empírica (notación de Hill):
C22H12N4Na4O13S4
Número de CAS:
Peso molecular:
760.57
Número MDL:
Código UNSPSC:
12171500
ID de la sustancia en PubChem:
NACRES:
NA.47

product name

Ponceau S solution, BioReagent, suitable (for use in cellulose acetate electrophoresis), 0.1 % (w/v) in 5% acetic acid

Línea del producto

BioReagent

Nivel de calidad

formulario

liquid

concentración

0.1 % (w/v) in 5% acetic acid

densidad

1.01 g/cm3 at 20 °C

idoneidad

suitable (for use in cellulose acetate electrophoresis)
suitable for electrophoresis

aplicaciones

diagnostic assay manufacturing
hematology
histology

temp. de almacenamiento

room temp

cadena SMILES

[Na+].[Na+].[Na+].[Na+].Oc1c(\N=N\c2ccc(cc2S([O-])(=O)=O)\N=N\c3ccc(cc3)S([O-])(=O)=O)c4ccc(cc4cc1S([O-])(=O)=O)S([O-])(=O)=O

InChI

1S/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;;;

Clave InChI

KQHKSGRIBYJYFX-WNDMICSESA-J

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Descripción general

Ponceau S is the sodium salt of a diazo dye. Ponceau S solution is a suitable reagent for use in electrophoresis studies. The Ponceau S stain comes ready-to-use and is designed for rapid (5 min) staining of protein bands on nitrocellulose or PVDF membranes (Western blots) and also for staining protein on cellulose acetate membranes. It may be employed as stain for the purified fibrinolytic enzyme (CK) for the analysis of N-terminal amino acid sequence of the first 14 residues of CK by a protein sequencer. Ponceau S staining on nitrocellulose and PVDF is reversible by washing the stained membrane with 0.1M NaOH for 1 minute. The stained and destained membrane can then be immunologically detected (Western blotting). A product information sheet is supplied which includes the staining procedure for cellulose acetate, nitrocellulose, and PVDF.

Aplicación

Ponceau S solution has been used for membrane staining (Western blotting).

Código de clase de almacenamiento

10 - Combustible liquids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

Not applicable

Punto de inflamabilidad (°C)

Not applicable


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Artículos

To meet the great diversity of protein analysis needs, Sigma offers a wide selection of protein visualization (staining) reagents. EZBlue™ and ProteoSilver™, designed specifically for proteomics, also perform impressively in traditional PAGE formats.

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