M8884

Methyl Green

~85% purity, powder

• Sinónimos: Ethyl Green
• Fórmula lineal: C₂₇H₃₅BrClN₃ · ZnCl₂
• Número de CAS: 7114-03-6
• Peso molecular: 653.24
• Número de índice de color: 42590
• Beilstein: 3914106
• Número CE: 230-415-4
• Número MDL: MFCD00151094
• Código UNSPSC: 12171500
• ID de la sustancia en PubChem: 24897293
• NACRES: NA.47

Propiedades

• Nombre del producto: Methyl Green, zinc chloride salt, ~85%
• Nivel de calidad: 200
• Ensayo: ~85%
• Formulario: powder
• composición: Dye Content, ≥83%
• técnicas: titration: suitable
• color: red to very dark red
• pH: 0.2-1.8, yellow to blue
• mp: >300 °C (lit.)
• solubilidad: water: 1 mg/mL
• εmax: >11100.00 at 417-425 nm in water at 0.005 g/L; >16000 at 311-319 nm in water at 0.005 g/L; >60000 at 626-634 nm in water at 0.005 g/L
• aplicaciones: diagnostic assay manufacturing; hematology; histology
• temp. de almacenamiento: room temp
• cadena SMILES: [Cl-].[Br-].Cl[Zn]Cl.CC[N+](C)(C)c1ccc(cc1)\C(c2ccc(cc2)N(C)C)=C3/C=C\C(C=C3)=[N+](\C)C
• InChI: 1S/C27H35N3.BrH.3ClH.Zn/c1-8-30(6,7)26-19-13-23(14-20-26)27(21-9-15-24(16-10-21)28(2)3)22-11-17-25(18-12-22)29(4)5;;;;;/h9-20H,8H2,1-7H3;4*1H;/q+2;;;;;+2/p-4
• Clave InChI: RRKSQWOQZFIQKW-UHFFFAOYSA-J

Descripción

Descripción general

Methyl green is a synthetic, triphenylmethane-type cationic dye related to ethyl green mainly used as DNA stain in histochemistry. It possesses aniline rings with different degrees of methylation. The dye binds specifically to the A-T rich regions of the major groove of DNA with the help of two positive charges. Methyl green in combination with pyronin is extensively used to stain RNA and plasma cells in tissue sections and cytologic preparations.

Aplicación

Methyl green is suitable as a nuclear counterstain in enzyme histochemistry, immunostaining, and in situ hybridization as the bluish-green color will be in contrast with red, purple, brown, and black products of histochemical reactions. It is also suitable as a tracking dye in Acid-polyacrylamide gel electrophoresis to separate gliadins. It has been used in the histochemical staining for the apoptosis examination of kidney tissue and as a nuclear stain for labeling embryos.

Calidad

Material may be a mixture of monobromo-monochloro and dichloro ZnCl₂ salts. Halogen contents listed on label.

Nota de análisis

Dye content based on molecular weight of monobromo-monochloro ZnCl₂ salt (653.2).

Información de seguridad

• Pictogramas: GHS05,GHS07,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H314,H335,H411
• Consejos de prudencia: P260 - P271 - P273 - P280 - P303 + P361 + P353 - P305 + P351 + P338
• Clasificaciones de peligro: Aquatic Chronic 2 - Eye Dam. 1 - Skin Corr. 1B - STOT SE 3
• Órganos de actuación: Respiratory system
• Código de clase de almacenamiento: 8A - Combustible corrosive hazardous materials
• Clase de riesgo para el agua (WGK): WGK 3
• Equipo de protección personal: dust mask type N95 (US), Eyeshields, Gloves