49940

ONPG Disks

suitable for microbiology

• Sinónimos: β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks
• Código UNSPSC: 41171621
• NACRES: NA.85

Propiedades

• Agency: according to GB 4789.4-2016
• Nivel de calidad: 100
• Línea del producto: BioChemika
• caducidad: limited shelf life, expiry date on the label
• técnicas: microbe id | specific enzyme detection: suitable
• aplicaciones: environmental; food and beverages; microbiology
• Condiciones de envío: wet ice
• temp. de almacenamiento: 2-8°C
• idoneidad: Escherichia coli; bacteria

Descripción

Descripción general

ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.

Envase

Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 3
• Punto de inflamabilidad (°F): Not applicable
• Punto de inflamabilidad (°C): Not applicable
• Equipo de protección personal: Eyeshields, Gloves, type N95 (US)