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Merck

30181

Supelco

Fluorescein sodium salt

analytical standard

Sinónimos:

Acid Yellow 73, D&C;Yellow No. 8, NaFl, NaFluo, Sodium fluorescein, Uranine

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About This Item

Fórmula empírica (notación de Hill):
C20H10Na2O5
Número de CAS:
Peso molecular:
376.27
Número de índice de color:
45350
Beilstein:
3833041
Número CE:
Número MDL:
Código UNSPSC:
85151701
ID de la sustancia en PubChem:
NACRES:
NA.24

grado

analytical standard

Nivel de calidad

Ensayo

≥97.5% (HPLC)

caducidad

limited shelf life, expiry date on the label

técnicas

HPLC: suitable
gas chromatography (GC): suitable

impurezas

≤15% water

fluorescencia

λex 460 nm; λem 515 nm(lit.)

Formato

neat

cadena SMILES

[Na+].[Na+].[O-]c1ccc2c(Oc3cc([O-])ccc3C24OC(=O)c5ccccc45)c1

InChI

1S/C20H12O5.2Na/c21-11-5-7-15-17(9-11)24-18-10-12(22)6-8-16(18)20(15)14-4-2-1-3-13(14)19(23)25-20;;/h1-10,21-22H;;/q;2*+1/p-2

Clave InChI

RGPLGPBQJOQFJS-UHFFFAOYSA-L

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Descripción general

Fluorescein sodium salt (uranine) is a xanthene dye, which finds applications as a fluorescent tracer and shows the best of fluorescence in the pH range 6 to 11. Uranine is also employed as a coloring additive in drugs and cosmetics. It is known to exhibit optical chemical sensing and fluorescent properties in biological staining procedures.

Aplicación

Fluorescein sodium salt is used as an analytical standard in determining the formation of hydroxy radicals, by the ZnO-based photocatalysts under UV-filtered simulated sunlight using spectrofluorometry. It is also used as a calibration standard for the label-free measurements of metabolic events within cells in the living animals using fluorescence lifetime imaging microscopy (FLIM).
It may also be used as an analytical reference standard for the quantification of the analyte in the following:
  • Soil samples using high-performance liquid chromatography (HPLC).
  • Coffee samples using high-performance liquid chromatography (HPLC) and capillary electrophoresis (CZE).

Refer to the product′s Certificate of Analysis for more information on a suitable instrument technique. Contact Technical Service for further support.

Nota de análisis

remarks on ICP: Na positive

Código de clase de almacenamiento

11 - Combustible Solids

Clase de riesgo para el agua (WGK)

WGK 1

Punto de inflamabilidad (°F)

423.7 °F - Pensky-Martens closed cup

Punto de inflamabilidad (°C)

217.6 °C - Pensky-Martens closed cup


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