RDLIG-RO
Roche
Rapid DNA Dephos & Ligation Kit
sufficient for 40 reactions (04898117001), sufficient for 160 reactions (04898125001), optimum reaction temp. 37 °C
Sinónimos:
cloning of dna fragments, dna ligase, ligases
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About This Item
uso
sufficient for 160 reactions (04898125001)
sufficient for 40 reactions (04898117001)
Nivel de calidad
fabricante / nombre comercial
Roche
Parámetros
37 °C optimum reaction temp.
temp. de almacenamiento
−20°C
Categorías relacionadas
Descripción general
The Rapid DNA Dephos & Ligation Kit enables fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments.
Especificidad
Specificity: rAPid Alkaline Phosphatase
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.
Alkaline Phosphatase catalyzes the hydrolysis of numerous phosphate esters, such as esters of primary and secondary alcohols, saccharides, cyclic alcohols, phenols and amines. Phosphodiesters do not react. The enzyme hydrolyzes inorganic pyrophosphate. The kinetic properties of the enzyme depend on many factors, such as purity of enzyme, concentration of enzyme in the assay, buffer, pH etc.
Heat inactivation: rApid Alkaline Phosphatase:
Two minutes at 75 °C.
T4 DNA Ligase:
10 minutes at 65 °C.
Aplicación
The Rapid DNA Dephos & Ligation Kit minimizes the time needed to perform diverse applications, including:
- Cloning of fragments into either plasmid or phage vectors
- Linker ligation
- Recircularization of linear vector DNA
- Generation of libraries
Envase
1 kit containing 5 components
Definición de unidad
Unit Definition: rAPid Alkaline Phosphatase
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.
Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl
One unit of rAPid Alkaline Phosphatase is the enzyme activity which hydrolyzes one μmol of 4-nitrophenyl phosphate in one minute at +37 °C under assay conditions.
Volume Activity: rApid Alkaline Phosphatase: 1 U/μl
T4 DNA Ligase: 5 U/μl
Otras notas
For life science research only. Not for use in diagnostic procedures.
Solo componentes del kit
Referencia del producto
Descripción
- rAPid Alkaline Phosphatase Buffer 10x concentrated
- rAPid Alkaline Phosphatase 1 U/μl
- DNA Dilution Buffer 5x concentrated
- T4 DNA Ligation Buffer 2x concentrated
- T4 DNA Ligase 5 U/μl
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
does not flash
Punto de inflamabilidad (°C)
does not flash
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