POLYDIC-RO
Roche
Poly[d(I-C)]
lyophilized, pkg of 10 U (10108812001 [A<sub>260</sub> units]), pkg of 50 U (11219847001 [A<sub>260</sub> units])
Sinónimos:
Poly-deoxy-inosinic-deoxy-cytidylic acid
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About This Item
Productos recomendados
descripción
Poly-deoxy-inosinic-deoxy-cytidylic acid
Nivel de calidad
Formulario
lyophilized
mol peso
28814 bp
envase
pkg of 10 U (10108812001 [A<sub>260</sub> units])
pkg of 50 U (11219847001 [A<sub>260</sub> units])
fabricante / nombre comercial
Roche
color
white
mp
54 °C (NaCl, 0.1 M, pH 7.0)
solubilidad
water: soluble
cociente de absorbancia
A290/260 nm 0.24-0.32
A280/260 nm 0.61-0.67
A250/260 nm 1.18-1.30
temp. de almacenamiento
−20°C
Aplicación
Poly[d(I-C)] is used as a template for RNA polymerases, and as a DNA model for biophysical investigations.
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).
It is also used as a primer template with Kornberg DNA polymerase for the determination of small amounts of dITP, dGTP, and dCTP. Poly(dI-dC) has been used in electrophoretic mobility shift assays (EMSA).
Características y beneficios
Contents
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence
Lyophilizate, sodium salt, base ratio 1:1, alternating base sequence
Calidad
Typical analysis:
Absorbance determination A250/A260, A280/A260, A290/A260
Absorbance determination A250/A260, A280/A260, A290/A260
Secuencia
Chain Length approx. 20,000 bp
In an aqueous solution the DNA itself is a salt (the acid groups of the phosphate back bone have a negative charge and are saturated by positive sodium ions) i.e. there is no additional salt in solution. The structural formula which shows the negative 0-groups in the phosphate backbone is available. The same number of positive sodium ions must be dissociated in solution as counter ions.
As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.
As the sodium ions are part of the product and saturate the phosphate groups, the lyophilisate is salt-free.
Definición de unidad
1 A260 unit corresponds to 50 μg ds DNA.
Unit Conversion: 1 A260 unit corresponds to 50 μg ds DNA.
Unit Conversion: 1 A260 unit corresponds to 50 μg ds DNA.
Nota de preparación
Activator: Polymines (spermine, spermidine)
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.
Storage conditions (working solution): Solution is stable to at least 12 months if stored at 2 to 8 °C, contact with DNase or bacteria is to avoid.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
11 - Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 1
Punto de inflamabilidad (°F)
Not applicable
Punto de inflamabilidad (°C)
Not applicable
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