11093657910
Roche
DIG DNA Labeling and Detection Kit
Sinónimos:
dna labeling and detection kit, dig
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About This Item
Código UNSPSC:
41105500
Productos recomendados
uso
sufficient for 25 labeling reactions
sufficient for 50 blots
Nivel de calidad
fabricante / nombre comercial
Roche
características de los productos alternativos más sostenibles
Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.
sustainability
Greener Alternative Product
categoría alternativa más sostenible
, Aligned
temp. de almacenamiento
−20°C
Descripción general
DIG DNA Labeling and Detection Kit is a convenient kit for random-primed labeling of DNA with digoxigenin-deoxyuridine triphosphate (dUTP), alkali-labile and color detection of hybrids by enzyme immunoassay. In this method, the complementary strand of denatured DNA is synthesized by Klenow polymerase using the 3′-OH termini of random oligonucleotides as primers.
Contents:
Contents:
- Unlabeled Control DNA 1, 100 μg/ml
- Unlabeled Control DNA 2, 200 μg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA, 5.2 μg/ml
- 10x Hexanucleotide Mix
- 10x dNTP Labeling Mixture
- Klenow Enzyme, Labeling grade, 2 U/μl
- Anti-digoxigenin-AP-conjugate, 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product is designed as a safer chemical. The DIG System was established as a sensitive and cost-effective alternative to radioactivity for the labeling and detection of nucleic acids. There are many available publications that prove the versatility of the DIG System, so use of radio-labeling is no longer the only option for labeling of DNA for hybridization.
Especificidad
Sensitivity and specificity: The standard labeling reaction with 1 μg template will give a yield of 260 ng DIG-labeled DNA after a 1-hour incubation at +37°C, or 780 ng after a 20-hour incubation. The sensitivity of DIG detection depends on the concentration of the DIG-labeled probe in the hybridization reaction, and the duration of the color reaction.
Aplicación
DIG DNA Labeling and Detection Kit has been used in a variety of hybridization techniques:
- Southern blots
- Northern blots
- Dot blots
- Colony and plaque screening
Envase
1 kit containing 10 components.
Nota de preparación
Working concentration: Dilution of Antibody 1:5,000
Otras notas
For life science research only. Not for use in diagnostic procedures.
Solo componentes del kit
Referencia del producto
Descripción
- Unlabeled Control DNA 1 100 µg/ml
- Unlabeled Control DNA 2 200 µg/ml
- DNA Dilution Buffer
- DIG-labeled Control DNA 5.2 µg/ml
- Hexanucleotide Mix 10x concentrated
- dNTP Labeling Mixture 10x concentrated
- Klenow Enzyme, Labeling grade 2 U/µl
- Anti-digoxigenin-AP-conjugate antibody 750 U/ml
- NBT/BCIP Concentrated Stock Solution
- Blocking Reagent
Ver todo (10)
Código de clase de almacenamiento
13 - Non Combustible Solids
Clase de riesgo para el agua (WGK)
WGK 3
Punto de inflamabilidad (°F)
does not flashNot applicable
Punto de inflamabilidad (°C)
does not flashNot applicable
Certificados de análisis (COA)
Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»
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Artículos
Digoxigenin (DIG) labeling methods and kits for DNA and RNA DIG probes, random primed DNA labeling, nick translation labeling, 5’ and 3’ oligonucleotide end-labeling.
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