10745782001
Roche
DNA, lambda
from bacteriophage lambda cl857 Sam 7
Sinónimos:
lambda dna
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About This Item
Código UNSPSC:
41106310
Productos recomendados
Nivel de calidad
Formulario
solution
mol peso
32,300 kDa
envase
pkg of 1 mL
fabricante / nombre comercial
Roche
condiciones de almacenamiento
avoid repeated freeze/thaw cycles
concentración
250 μg/mL
impurezas
RNA, none detected
nuclease, none detected
color
colorless
solubilidad
water: miscible
Condiciones de envío
dry ice
temp. de almacenamiento
−20°C (−15°C to −25°C)
Descripción general
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
Aplicación
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Calidad
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Secuencia
Chain Length 48,502 bp
Forma física
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Almacenamiento y estabilidad
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
Otras notas
For life science research only. Not for use in diagnostic procedures.
Código de clase de almacenamiento
12 - Non Combustible Liquids
Clase de riesgo para el agua (WGK)
nwg
Punto de inflamabilidad (°F)
No data available
Punto de inflamabilidad (°C)
No data available
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