10354074001

Citrate Lyase (CL)

from Klebsiella pneumoniae (Aerobacter aerogenes)

• Sinónimos: Citrate Lyase (CL)
• Comisión internacional de enzimas: 4.1.3.6 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: bacterial (Klebsiella pneumoniae)
• Nivel de calidad: 100
• Formulario: lyophilized
• actividad específica: ≥0.25 U/mg (At 25 °C with citrate as the substrate.)
• envase: pkg of 120 U
• fabricante / nombre comercial: Roche
• técnicas: activity assay: suitable
• color: light cream
• pH óptimo: 8.0-9.0
• solubilidad: water: soluble
• Nº de acceso NCBI: WP_323133469
• Nº de acceso UniProt: P45413
• aplicaciones: life science and biopharma
• actividad extraña: ICDH <0.05% (NAD as coenzyme); NADH oxidase <0.05%
• temp. de almacenamiento: 2-8°C

Descripción

Descripción general

Citrate lyase is considered as a substrate- induced enzyme which helps in catalyzing reversible aldol cleavage of citrate to oxaloacetate and acetate. The enzyme is adaptive to anaerobic dissimilation of citrate.

Aplicación

It was used in enzymatic assay to measure citrate concentration using microtiter plates.

Calidad

Contaminants: <0.05% ICDH (NAD specific) and “NADH oxidase”, each

Forma física

Lyophilizate, stabilized with BSA, sucrose, MgSO₄ and EDTA. Note: A solution in double-distilled water has a pH-value of approximately 7.0. The enzyme can be relyophilized.

Nota de preparación

Activator: Mg²⁺ (K_m = 3.0 mM, pH 7.4, 25 °C); other divalent metal ions (Mn²⁺, Fe²⁺, Zn²⁺)

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash