10109339001

Sorbitol Dehydrogenase (SDH)

from sheep liver

• Sinónimos: SDH, Sorbitol Dehydrogenase, sorbitol
• Comisión internacional de enzimas: 1.1.1.14 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propiedades

• origen biológico: sheep liver
• Nivel de calidad: 100
• formulario: lyophilized
• actividad específica: ~40 units/mg protein (At 25 °C with D-fructose as the substrate.)
• mol peso: 115 kDa
• envase: pkg of 10 mg (60 mg lyophilizate)
• fabricante / nombre comercial: Roche
• concentración: 0.16 mg/mL Biuret
• técnicas: activity assay: suitable
• color: white
• pH óptimo: 7.5
• solubilidad: water: 60 mg/mL, clear, colorless
• idoneidad: suitable for ELISA applications
• Nº de acceso NCBI: XP_004010700
• Nº de acceso UniProt: P07846
• aplicaciones: life science and biopharma
• actividad extraña: ADH <0.00182%; GIDH <0.00152%; Glucose-DH <0.00182% (NAD as coenz.); LDH <0.00167%; MDH <0.00198%; NADH oxidase 0.00532%
• temp. de almacenamiento: 2-8°C
• Información sobre el gen: sheep ... SORD(101115280)

Descripción

Descripción general

L-iditol:NAD⁺ 5′-oxidoreductase
Sorbitol dehydrogenase (SDH) from sheep liver has a molar mass of 152kDa. The protein is a tetramer of four identical subunits. Each of these subunits has 355 amino acid residues, of which 10 are cysteine residues. Each subunit contains a zinc atom at the active site, which is associated with three protein ligands and a water molecule. The zinc atom associates with the oxygen of the sorbitol hydroxyl or of the fructose carbonyl interconverted during catalysis.

Aplicación

Reduces L-iditol to L-sorbose. Also acts on D-glucitol and other closely related sugar alcohols. Allows the reduction of ketones to polyols (see aldolases for the synthesis of ketoses).

Acciones bioquímicas o fisiológicas

Sorbitol dehydrogenase (SDH) catalyzes the reversible NAD-linked conversion of D-sorbitol to D-fructose and forms a part of the sorbitol pathway, which is a crucial bypass to glycolysis for glucose metabolism. This pathway has been found to be associated with the accumulation of sorbitol in lens, which results in diabetic cataractogenesis. SDH also oxidizes various polyols and other secondary alcohols into their corresponding ketones.

Calidad

Contaminants: <0.01% ADH, <0.02% GIDH and glucose dehydrogenase, each, <0.05% LDH and MDH, each.

Definición de unidad

One unit (U) sorbitol dehydrogenase will reduce 1 mol of D-fructose in one minute at +25 °C and pH 7.6 [triethanolamine buffer; 150 mM fructose (nonsaturating concentration)]. The above assay consumes 1 mol of NADH per mol of D-sorbitol formed.

Forma física

Lyophilizate (60 mg contain 10 mg enzyme protein and 50 mg maltose).

Nota de preparación

Activator: The reactions (oxidation or reduction) are fastest in Tris or triethanolamine buffer.
Stabilizers: Maltose is used as stabilizer.
Storage conditions (working solution): An aqueous solution is stable at 2 to 8 °C for several weeks.

Almacenamiento y estabilidad

Store at 2 to 8 °C. (Store the lyophilizate dry.)

Otras notas

For life science research only. Not for use in diagnostic procedures.

Información de seguridad

• Código de clase de almacenamiento: 11 - Combustible Solids
• Clase de riesgo para el agua (WGK): WGK 1
• Punto de inflamabilidad (°F): does not flash
• Punto de inflamabilidad (°C): does not flash