PRCYTMAG40K-PX24

MILLIPLEX^® Non-Human Primate Cytokine/Chemokine Panel II - 24-Plex Premixed Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in non-human primate serum, plasma and cell culture samples.

• Código UNSPSC: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Propiedades

• Nivel de calidad: 200
• reactividad de especies: nonhuman primates
• fabricante / nombre comercial: Milliplex^®
• assay range: standard curve range: 1-1,500 pg/mL; (IL-17A); standard curve range: 10-10,000 pg/mL; (sCD137, IL-2, IP-10/CXCL10); standard curve range: 146-150,000 pg/mL; (IL-4); standard curve range: 15-15,000 pg/mL; (IL-21, IL-31); standard curve range: 20-20,000 pg/mL; (FGF-2, IL-33); standard curve range: 24-25,000 pg/mL; (IL-23); standard curve range: 29-30,000 pg/mL; (IL-1α, IL-28A); standard curve range: 293-300,000 pg/mL; (IL-17E/IL-25); standard curve range: 3-3,000 pg/mL; (Eotaxin/CCL11, IL-6); standard curve range: 4-4,000 pg/mL; (Granzyme B); standard curve range: 7-7,000 pg/mL; (MIP-3α/CCL20); standard curve range: 73-75,000 pg/mL; (IL-16); standard curve range: 78-80,000 pg/mL; (Granzyme A); standard curve range: 8-8,000 pg/mL; (sFasL); standard curve range: 98-100,000 pg/mL; (Fractalkine/CX3CL1, IL-22, Perforin, TNFβ/LTA)
• técnicas: multiplexing: suitable
• compatibilidad: configured for Premixed
• método de detección: fluorometric (Luminex xMAP)
• Condiciones de envío: wet ice

Descripción

Descripción general

Cytokines, immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses, are one of the regulatory mechanisms of the immune system. Acting at the recognition, activation or effector phases of an immune response, cytokines modulate the development and functional activities of the subtypes of T cells, B cells and myeloid cells. Since chronic inflammation and aberrant immune response contribute to many disease states, research involving cytokines and chemokines plays a significant role in achieving a deeper understanding of the etiology and progression of diseases such as infectious disease, autoimmune and chronic inflammatory disease, cardiovascular disease, metabolic syndrome, neurological disorders and cancer.

The MILLIPLEX^® Non-Human Primate Premixed Cytokine Panel II is a 25 plex kit to be used for the simultaneous quantification of the following analytes in monkey serum, plasma, tissue/cell lysate and culture supernatant samples: sCD137, Eotaxin, sFASL, FGF-2, Fractalkine, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10, MIP-3α, Perforin, RANTES and TNFβ.

The Luminex^® xMAP^® platform uses a magnetic bead immunoassay format for ideal speed and sensitivity to quantitate multiple analytes simultaneously, dramatically improving productivity while conserving valuable sample volume.

Panel Type: Cytokines/Chemokines

Especificidad

Cross Reactivty
This multiplexed assay panel has been tested with samples from multiple non-human primate species. Please see protocol for details.

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Aplicación

• Analytes: sCD137, Eotaxin/CCL11, sFasL, FGF-2, Fractalkine/CX3CL1, Granzyme A, Granzyme B, IL-1α, IL-2, IL-4, IL-6, IL-16, IL-17A, IL-17E/IL-25, IL-21, IL-22, IL-23, IL-28A, IL-31, IL-33, IP-10/CXCL10, MIP-3α/CCL20, Perforin, TNFβ/LTA
• Recommended Sample type: serum, plasma and cell culture samples
• Recommended Sample dilution: 1:2 diluted plasma or serum
• Assay Run Time: Overnight or one day. For best results, an overnight incubation is recommended
• Research Category: Inflammation & Immunology

Componentes

Non-Human Primate Cytokine Panel 2 Standard, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 1, 1 vial, lyophilized

Non-Human Primate Cytokine Panel 2 Quality Control 2, 1 vial, lyophilized

Serum Matrix (0.08% Sodium Azide), 1 bottle, lyophilized

Sample Plate, 96 well format, 1 plate

2 Foil plate sealers

Assay Buffer, 2 bottles, 30 mL

Bead Diluent, 1 bottle, 3.5 mL

Wash Buffer, 10X (0.05% Proclin), 2 bottlesl, 30 mL

Non-Human Primate Cytokine Panel 2 Detection Antibodies, 1 bottle, 3.2 mL

Streptavidin-Phycoerythrin, 1 bottle, 3.2 mL

Premixed 24-plex Beads, 1 bottle, 3.5 mL

Almacenamiento y estabilidad

Recommended storage for kit components is 2 - 8°C.

Otras notas

Sensitivity: Refer to kit protocol for sensitivities of individual biomarkers.

Información legal

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Cláusula de descargo de responsabilidad

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Información de seguridad

• Pictogramas: GHS06,GHS08,GHS05,GHS09
• Palabra de señalización: Danger
• Frases de peligro: H302 + H332,H311,H317,H318,H373,H411
• Consejos de prudencia: P273 - P280 - P302 + P352 + P312 - P304 + P340 + P312 - P305 + P351 + P338 - P314
• Clasificaciones de peligro: Acute Tox. 3 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
• Órganos de actuación: Respiratory Tract
• Código de clase de almacenamiento: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects