PK1109

PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb

liquid, Calbiochem^®

• Código UNSPSC: 12352203

Propiedades

• origen biológico: rabbit
• Nivel de calidad: 100
• forma del anticuerpo: purified antibody
• tipo de anticuerpo: primary antibodies
• clon: polyclonal
• formulario: liquid
• no contiene: preservative
• reactividad de especies: mouse, rat, chicken, human, Xenopus
• fabricante / nombre comercial: Calbiochem^®
• condiciones de almacenamiento: OK to freeze
• isotipo: IgG
• Condiciones de envío: wet ice
• temp. de almacenamiento: −20°C
• modificación del objetivo postraduccional: phosphorylation (pTyr416)
• Información sobre el gen: human ... SRC(6714)

Descripción

Descripción general

Protein A and immunoaffinity purified rabbit polyclonal antibody. Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁶.

Recognizes the ~60 kDa Src protein phosphorylated at Tyr⁴¹⁶ in COLO 201 cells and PDGF-treated NIH/3T3 cells. May also recognize other Src family kinases phosphorylated at equivalent sites.

This PhosphoDetect Anti-Src Family (pTyr⁴¹⁶) Rabbit pAb is validated for use in Immunoblotting for the detection of Src Family (pTyr⁴¹⁶).

Inmunógeno

Human

a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr⁴¹⁶ phosphorylation site of human Src

Aplicación

Immunoblotting (1:1000)

Advertencia

Toxicity: Standard Handling (A)

Forma física

In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.

Reconstitución

Do not aliquot.

Nota de análisis

Positive Control
COLO 201 cells, PDGF-treated NIH/3T3 cells, and breast carcinoma

Otras notas

May also recognize other Src family kinases phosphorylated at equivalent sites. The immunogen sequence is 100% conserved in chicken, rat, and Xenopus, but cross-reactivity has not been tested. For immunoblotting the membrane should be incubated with antibody diluted in 5% BSA, 1X TBS, 0.1% Tween^®-20 detergent at 4°C overnight with gentle shaking. Antibody should be titrated for optimal results in individual systems.

Thomas, S.M. and Brugge, J.S. 1997. Annu. Rev. Cell Dev. Biol.13, 513.
Chow, L.M. et al. 1993. Nature365, 156.
Molina, T.J., et al. 1992. Nature357, 161.
Straus, D.B. and Weiss, A. 1992. Cell70, 585.
Yamanashi, Y., et al. 1991. Science251, 192.
Yamanashi, Y., et al. 1989. Proc. Natl. Acad. Sci. USA86, 6538.
Hunter, T. 1987. Cell49, 1.

Información legal

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

TWEEN is a registered trademark of Croda International PLC

Información de seguridad

• Código de clase de almacenamiento: 10 - Combustible liquids
• Clase de riesgo para el agua (WGK): WGK 2